8sgy
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[8sgy]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_tarentolae Leishmania tarentolae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8SGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8SGY FirstGlance]. <br> | <table><tr><td colspan='2'>[[8sgy]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_tarentolae Leishmania tarentolae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8SGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8SGY FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTI:5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL'>BTI</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.62Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTI:5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL'>BTI</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8sgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8sgy OCA], [https://pdbe.org/8sgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8sgy RCSB], [https://www.ebi.ac.uk/pdbsum/8sgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8sgy ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8sgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8sgy OCA], [https://pdbe.org/8sgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8sgy RCSB], [https://www.ebi.ac.uk/pdbsum/8sgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8sgy ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/A0A640KC69_LEITA A0A640KC69_LEITA] | [https://www.uniprot.org/uniprot/A0A640KC69_LEITA A0A640KC69_LEITA] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Propionyl-CoA carboxylase (PCC) is a multienzyme complex consisting of up to six alpha-subunits and six beta-subunits. Belonging to a metabolic pathway converging on the citric acid cycle, it is present in most forms of life and irregularities in its assembly lead to serious illness in humans, known as propionic acidemia. Here, we report the cryogenic electron microscopy (cryoEM) structures and assembly of different oligomeric isomers of endogenous PCC from the parasitic protozoan Leishmania tarentolae (LtPCC). These structures and their statistical distribution reveal the mechanics of PCC assembly and disassembly at equilibrium. We show that, in solution, endogenous LtPCC beta-subunits form stable homohexamers, to which different numbers of alpha-subunits attach. Sorting LtPCC particles into seven classes (i.e., oligomeric formulae alpha(0)beta(6), alpha(1)beta(6), alpha(2)beta(6), alpha(3)beta(6), alpha(4)beta(6), alpha(5)beta(6), alpha(6)beta(6)) enables formulation of a model for PCC assembly. Our results suggest how multimerization regulates PCC enzymatic activity and showcase the utility of cryoEM in revealing the statistical mechanics of reaction pathways. | ||
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| - | CryoEM reveals oligomeric isomers of a multienzyme complex and assembly mechanics.,Lee JKJ, Liu YT, Hu JJ, Aphasizheva I, Aphasizhev R, Zhou ZH J Struct Biol X. 2023 Apr 8;7:100088. doi: 10.1016/j.yjsbx.2023.100088. , eCollection 2023. PMID:37128595<ref>PMID:37128595</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 8sgy" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Leishmania tarentolae propionyl-CoA carboxylase (alpha-5-beta-6)
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