1srp
From Proteopedia
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'''STRUCTURAL ANALYSIS OF SERRATIA PROTEASE''' | '''STRUCTURAL ANALYSIS OF SERRATIA PROTEASE''' | ||
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[[Category: Katsube, Y.]] | [[Category: Katsube, Y.]] | ||
[[Category: Katsuya, Y.]] | [[Category: Katsuya, Y.]] | ||
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Revision as of 06:04, 3 May 2008
STRUCTURAL ANALYSIS OF SERRATIA PROTEASE
Overview
The crystal structure of Serratia protease from Serratia sp. E-15 was solved by the single isomorphous replacement method supplemented with anomalous scattering effects from both the Zn atom in the native crystal and the Sm atom in the derivative crystal, and refined at 2.0 A resolution to a crystallographic R-factor of 0.194. The enzyme consists of N-terminal catalytic and C-terminal beta-sandwich domains, as observed in alkaline protease from Pseudomonas aeruginosa IFO3080. The catalytic domain with a five-stranded antiparallel beta-sheet and five alpha-helices shares a basically common folding topology with those of other zinc metalloendoproteases. The catalytic zinc ion at the bottom of the active site cleft is ligated by His176, His180, His186, Tyr216, and a water molecule in a distorted trigonalbipyramidal manner. The C-terminal domain is a beta-strand-rich domain containing eighteen beta-strands and a short alpha-helix, and has seven Ca2+ ions bound to calcium binding loops. An unusual beta-sheet coil motif is observed in this domain, where the beta-strands and calcium binding loops are alternately incorporated into an elliptical right-handed spiral so as to form a two-layer untwisted beta-sandwich structure. The Ca2+ ions in the C-terminal domain seem to be very important for the folding and stability of the beta-sheet coil structure.
About this Structure
1SRP is a Single protein structure of sequence from Serratia sp.. Full crystallographic information is available from OCA.
Reference
Crystal structure of Serratia protease, a zinc-dependent proteinase from Serratia sp. E-15, containing a beta-sheet coil motif at 2.0 A resolution., Hamada K, Hata Y, Katsuya Y, Hiramatsu H, Fujiwara T, Katsube Y, J Biochem. 1996 May;119(5):844-51. PMID:8797082 Page seeded by OCA on Sat May 3 09:04:01 2008
