8rvc

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Current revision (07:17, 3 July 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 8rvc is ON HOLD until Paper Publication
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==Crystal structure of alpha keto acid C-methyl-transferases MrsA bound to ketoarginine==
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<StructureSection load='8rvc' size='340' side='right'caption='[[8rvc]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8rvc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RVC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.969&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NWG:5-[(diaminomethylidene)amino]-2-oxopentanoic+acid'>NWG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rvc OCA], [https://pdbe.org/8rvc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rvc RCSB], [https://www.ebi.ac.uk/pdbsum/8rvc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rvc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MRSA_PSESY MRSA_PSESY]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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S‑adenosyl-l-methionine-dependent methyltransferases (MTs) are involved in the C-methylation of a variety of natural products. The MTs SgvM from Streptomyces griseoviridis and MrsA from Pseudomonas syringae pv. syringae catalyze the methylation of the beta-carbon atom of alpha-keto acids in the biosynthesis of the antibiotic natural products viridogrisein and 3‑methylarginine, respectively. MrsA shows high substrate selectivity for 5‑guanidino-2-oxovalerate, while other alpha-keto acids, such as the SgvM substrates 4-methyl-2-oxovalerate, 2-oxovalerate, and phenylpyruvate, are not accepted. Here we report the crystal structures of SgvM and MrsA in the apo form bound with substrate or S‑adenosyl-l-methionine. By investigating key residues for substrate recognition in the active sites of both enzymes and engineering MrsA by site-directed mutagenesis, the substrate range of MrsA was extended to accept alpha‑keto acid substrates of SgvM with uncharged and lipophilic beta‑residues. Our results showcase the transfer of the substrate scope of alpha-keto acid MTs from different biosynthetic pathways by rational design.
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Authors: Gerhardt, S., Kemper, F., Andexer, J.N.
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Structures and protein engineering of the alpha-keto acid C-methyltransferases SgvM and MrsA for rational substrate transfer.,Sommer-Kamann C, Breiltgens J, Zou Z, Gerhardt S, Saleem-Batcha R, Kemper F, Einsle O, Andexer JN, Muller M Chembiochem. 2024 Jun 18:e202400258. doi: 10.1002/cbic.202400258. PMID:38887142<ref>PMID:38887142</ref>
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Description: Crystal structure of alpha keto acid C-methyl-transferases MrsA bound to ketoarginine
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Andexer, J.N]]
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<div class="pdbe-citations 8rvc" style="background-color:#fffaf0;"></div>
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[[Category: Kemper, F]]
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== References ==
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[[Category: Gerhardt, S]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pseudomonas syringae]]
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[[Category: Andexer JN]]
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[[Category: Gerhardt S]]
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[[Category: Kemper F]]

Current revision

Crystal structure of alpha keto acid C-methyl-transferases MrsA bound to ketoarginine

PDB ID 8rvc

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