7yoj
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7yoj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Armatimonadetes_bacterium Armatimonadetes bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YOJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YOJ FirstGlance]. <br> | <table><tr><td colspan='2'>[[7yoj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Armatimonadetes_bacterium Armatimonadetes bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YOJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YOJ FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yoj OCA], [https://pdbe.org/7yoj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yoj RCSB], [https://www.ebi.ac.uk/pdbsum/7yoj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yoj ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.36Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yoj OCA], [https://pdbe.org/7yoj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yoj RCSB], [https://www.ebi.ac.uk/pdbsum/7yoj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yoj ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | [https://www.uniprot.org/uniprot/ | + | [https://www.uniprot.org/uniprot/A0A399WQY8_UNCAM A0A399WQY8_UNCAM] |
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
CRISPR-Cas modules serve as the adaptive nucleic acid immune systems for prokaryotes, and provide versatile tools for nucleic acid manipulation in various organisms. Here, we discovered a new miniature type V system, CRISPR-Caspi (Cas12l) (~860 aa), from the environmental metagenome. Complexed with a large guide RNA (~170 nt) comprising the tracrRNA and crRNA, Caspi (Cas12l) recognizes a unique 5' C-rich PAM for DNA cleavage under a broad range of biochemical conditions, and generates gene editing in mammalian cells. Cryo-EM study reveals a 'bracelet' architecture of Caspi effector encircling the DNA target at 3.4 A resolution, substantially different from the canonical 'two-lobe' architectures of Cas12 and Cas9 nucleases. The large guide RNA serves as a 'two-arm' scaffold for effector assembly. Our study expands the knowledge of DNA targeting mechanisms by CRISPR effectors, and offers an efficient but compact platform for DNA manipulation. | CRISPR-Cas modules serve as the adaptive nucleic acid immune systems for prokaryotes, and provide versatile tools for nucleic acid manipulation in various organisms. Here, we discovered a new miniature type V system, CRISPR-Caspi (Cas12l) (~860 aa), from the environmental metagenome. Complexed with a large guide RNA (~170 nt) comprising the tracrRNA and crRNA, Caspi (Cas12l) recognizes a unique 5' C-rich PAM for DNA cleavage under a broad range of biochemical conditions, and generates gene editing in mammalian cells. Cryo-EM study reveals a 'bracelet' architecture of Caspi effector encircling the DNA target at 3.4 A resolution, substantially different from the canonical 'two-lobe' architectures of Cas12 and Cas9 nucleases. The large guide RNA serves as a 'two-arm' scaffold for effector assembly. Our study expands the knowledge of DNA targeting mechanisms by CRISPR effectors, and offers an efficient but compact platform for DNA manipulation. | ||
| - | The compact Caspi (Cas12l) 'bracelet' provides a unique structural platform for DNA manipulation.,Sun A, Li CP, Chen Z, Zhang S, Li DY, Yang Y, Li LQ, Zhao Y, Wang K, Li Z, Liu J, Liu S, Wang J, Liu JG Cell Res. 2023 | + | The compact Caspi (Cas12l) 'bracelet' provides a unique structural platform for DNA manipulation.,Sun A, Li CP, Chen Z, Zhang S, Li DY, Yang Y, Li LQ, Zhao Y, Wang K, Li Z, Liu J, Liu S, Wang J, Liu JG Cell Res. 2023 Mar;33(3):229-244. doi: 10.1038/s41422-022-00771-2. Epub 2023 Jan , 17. PMID:36650285<ref>PMID:36650285</ref> |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
Current revision
Structure of CasPi with guide RNA and target DNA
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