8i54
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[8i54]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lachnospiraceae_bacterium_MA2020 Lachnospiraceae bacterium MA2020] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I54 FirstGlance]. <br> | <table><tr><td colspan='2'>[[8i54]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lachnospiraceae_bacterium_MA2020 Lachnospiraceae bacterium MA2020] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I54 FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i54 OCA], [https://pdbe.org/8i54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i54 RCSB], [https://www.ebi.ac.uk/pdbsum/8i54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i54 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.95Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i54 OCA], [https://pdbe.org/8i54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i54 RCSB], [https://www.ebi.ac.uk/pdbsum/8i54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i54 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cas12a is a programmable nuclease for adaptive immunity against invading nucleic acids in CRISPR-Cas systems. Here, we report the crystal structures of apo Cas12a from Lachnospiraceae bacterium MA2020 (Lb2) and the Lb2Cas12a+crRNA complex, as well as the cryo-EM structure and functional studies of the Lb2Cas12a+crRNA+DNA complex. We demonstrate that apo Lb2Cas12a assumes a unique, elongated conformation, whereas the Lb2Cas12a+crRNA binary complex exhibits a compact conformation that subsequently rearranges to a semi-open conformation in the Lb2Cas12a+crRNA+DNA ternary complex. Notably, in solution, apo Lb2Cas12a is dynamic and can exist in both elongated and compact forms. Residues from Met493 to Leu523 of the WED domain undergo major conformational changes to facilitate the required structural rearrangements. The REC lobe of Lb2Cas12a rotates 103 degrees concomitant with rearrangement of the hinge region close to the WED and RuvC II domains to position the RNA-DNA duplex near the catalytic site. Our findings provide insight into crRNA recognition and the mechanism of target DNA cleavage. | ||
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| + | Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage.,Jianwei L, Jobichen C, Machida S, Meng S, Read RJ, Hongying C, Jian S, Yuan YA, Sivaraman J PLoS Biol. 2023 Mar 14;21(3):e3002023. doi: 10.1371/journal.pbio.3002023. , eCollection 2023 Mar. PMID:36917574<ref>PMID:36917574</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 8i54" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Lb2Cas12a RNA DNA complex
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