Journal:Acta Cryst D:S2059798324005461
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<StructureSection load='' size='450' side='right' scene='10/1050894/Cv/1' caption=''>__NOTOC__ | <StructureSection load='' size='450' side='right' scene='10/1050894/Cv/1' caption=''>__NOTOC__ | ||
===Toward a dependable data set of structures for L-asparaginase=== | ===Toward a dependable data set of structures for L-asparaginase=== | ||
| - | <big>Alexander Wlodawer, Zbigniew Dauter, Jacek Lubkowski, Joanna I. Loch, Dariusz Brzezinski, Miroslaw Gilski, Mariusz Jaskolski</big> <ref>Wlodawer, A. ''et al.'' "Toward a dependable dataset of structures for L-asparaginase research", ''Acta Crystallogr D Struct Biol'', 80:50-527 (2024). [https://journals.iucr.org/paper?S2059798324005461 DOI:10.1107/S2059798324005461]</ref> | + | <big>Alexander Wlodawer, Zbigniew Dauter, Jacek Lubkowski, Joanna I. Loch, Dariusz Brzezinski, Miroslaw Gilski, Mariusz Jaskolski</big> <ref name="dependable">Wlodawer, A. ''et al.'' "Toward a dependable dataset of structures for L-asparaginase research", ''Acta Crystallogr D Struct Biol'', 80:50-527 (2024). [https://journals.iucr.org/paper?S2059798324005461 DOI:10.1107/S2059798324005461]</ref> |
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==Structural studies== | ==Structural studies== | ||
| - | The first L-asparaginase structure was published and deposited in the PDB in 1993 for the EcAII enzyme<ref>PMID:8434007</ref> and may serve as an example of a Class 1 type II enzyme ('''Figure EcAII_3eca'''). Structure of Class 1 type I enzymes is exemplified by EcAI<ref>PMID:17451745</ref> ('''Existing Figure'''), whereas Class 2 type III enzymes may be represented by EcAIII<ref>PMID:15159592</ref><ref>PMID:18334484</ref> ('''Figure EcAIII_2zal'''). Class 2 L-asparaginases belong to the family of Ntn-hydrolases, which are expressed as inactive precursors that must undergo autoproteolytic cleavage into α and β subunits to achieve maturation<ref>PMID:35626629</ref>. While the existence of an alien type of ASNase in the symbiotic nitrogen-fixing bacterium Rhizobium etli had been recognized long ago<ref>PMID:11996000</ref>, the structure of the inducible and thermolabile prototype Class 3 ReAV was solved and deposited in the PDB only recently<ref>PMID:34795296</ref> ('''Figure ReAV_7os5'''), followed by structures of the constitutive and thermostable isoform ReAIV<ref>PMID:37494066</ref>. More than 200 structures of ASNases have been deposited in the Protein Data Bank (PDB) by April 2024<ref>PMID:34060231</ref><ref | + | The first L-asparaginase structure was published and deposited in the PDB in 1993 for the EcAII enzyme<ref>PMID:8434007</ref> and may serve as an example of a Class 1 type II enzyme ('''Figure EcAII_3eca'''). Structure of Class 1 type I enzymes is exemplified by EcAI<ref>PMID:17451745</ref> ('''Existing Figure'''), whereas Class 2 type III enzymes may be represented by EcAIII<ref>PMID:15159592</ref><ref>PMID:18334484</ref> ('''Figure EcAIII_2zal'''). Class 2 L-asparaginases belong to the family of Ntn-hydrolases, which are expressed as inactive precursors that must undergo autoproteolytic cleavage into α and β subunits to achieve maturation<ref>PMID:35626629</ref>. While the existence of an alien type of ASNase in the symbiotic nitrogen-fixing bacterium Rhizobium etli had been recognized long ago<ref>PMID:11996000</ref>, the structure of the inducible and thermolabile prototype Class 3 ReAV was solved and deposited in the PDB only recently<ref>PMID:34795296</ref> ('''Figure ReAV_7os5'''), followed by structures of the constitutive and thermostable isoform ReAIV<ref>PMID:37494066</ref>. More than 200 structures of ASNases have been deposited in the Protein Data Bank (PDB) by April 2024<ref>PMID:34060231</ref><ref name="dependable"/>. |
==Dependable Structures== | ==Dependable Structures== | ||
Revision as of 12:51, 11 July 2024
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