7b5p
From Proteopedia
(Difference between revisions)
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==AcrB in cycloalkane amphipol== | ==AcrB in cycloalkane amphipol== | ||
| - | <StructureSection load='7b5p' size='340' side='right'caption='[[7b5p]]' scene=''> | + | <StructureSection load='7b5p' size='340' side='right'caption='[[7b5p]], [[Resolution|resolution]] 3.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B5P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B5P FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B5P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B5P FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b5p OCA], [https://pdbe.org/7b5p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b5p RCSB], [https://www.ebi.ac.uk/pdbsum/7b5p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b5p ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b5p OCA], [https://pdbe.org/7b5p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b5p RCSB], [https://www.ebi.ac.uk/pdbsum/7b5p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b5p ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Membrane proteins are essential for cellular growth, signalling and homeostasis, making up a large proportion of therapeutic targets. However, the necessity for a solubilising agent to extract them from the membrane creates challenges in their structural and functional study. Although amphipols have been very effective for single-particle electron cryo-microscopy (cryoEM) and mass spectrometry, they rely on initial detergent extraction before exchange into the amphipol environment. Therefore, circumventing this pre-requirement would be a big advantage. Here we use an alternative type of amphipol: a cycloalkane-modified amphiphile polymer (CyclAPol) to extract Escherichia coli AcrB directly from the membrane and demonstrate that the protein can be isolated in a one-step purification with the resultant cryoEM structure achieving 3.2 A resolution. Together this work shows that cycloalkane amphipols provide a powerful approach for the study of membrane proteins, allowing native extraction and high-resolution structure determination by cryoEM. | ||
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| + | Cycloalkane-modified amphiphilic polymers provide direct extraction of membrane proteins for CryoEM analysis.,Higgins AJ, Flynn AJ, Marconnet A, Musgrove LJ, Postis VLG, Lippiat JD, Chung CW, Ceska T, Zoonens M, Sobott F, Muench SP Commun Biol. 2021 Nov 25;4(1):1337. doi: 10.1038/s42003-021-02834-3. PMID:34824357<ref>PMID:34824357</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7b5p" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
AcrB in cycloalkane amphipol
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