7bfq
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Structure of the Integrator cleavage module with extended INTS4 and rigid body docked INTS9/11 CTD== |
| - | <StructureSection load='7bfq' size='340' side='right'caption='[[7bfq]]' scene=''> | + | <StructureSection load='7bfq' size='340' side='right'caption='[[7bfq]], [[Resolution|resolution]] 4.15Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7bfq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BFQ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bfq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bfq OCA], [https://pdbe.org/7bfq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bfq RCSB], [https://www.ebi.ac.uk/pdbsum/7bfq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bfq ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.15Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bfq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bfq OCA], [https://pdbe.org/7bfq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bfq RCSB], [https://www.ebi.ac.uk/pdbsum/7bfq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bfq ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/INT9_HUMAN INT9_HUMAN] Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Integrator is a specialized 3' end-processing complex involved in cleavage and transcription termination of a subset of nascent RNA polymerase II transcripts, including small nuclear RNAs (snRNAs). We provide evidence of the modular nature of the Integrator complex by biochemically characterizing its two subcomplexes, INTS5/8 and INTS10/13/14. Using cryoelectron microscopy (cryo-EM), we determined a 3.5-A-resolution structure of the INTS4/9/11 ternary complex, which constitutes Integrator's catalytic core. Our structure reveals the spatial organization of the catalytic nuclease INTS11, bound to its catalytically impaired homolog INTS9 via several interdependent interfaces. INTS4, a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. In this assembly, all three proteins form a composite electropositive groove, suggesting a putative RNA binding path within the complex. Comparison with other 3' end-processing machineries points to distinct features and a unique architecture of the Integrator's catalytic module. | ||
| + | |||
| + | Structure of the catalytic core of the Integrator complex.,Pfleiderer MM, Galej WP Mol Cell. 2021 Mar 18;81(6):1246-1259.e8. doi: 10.1016/j.molcel.2021.01.005. Epub , 2021 Feb 5. PMID:33548203<ref>PMID:33548203</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bfq" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Integrator complex 3D structures|Integrator complex 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Galej WP]] |
| + | [[Category: Pfleiderer MM]] | ||
Current revision
Structure of the Integrator cleavage module with extended INTS4 and rigid body docked INTS9/11 CTD
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