7o4h
From Proteopedia
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| - | ==== | + | ==The structure of the native CNGA1/CNGB1 CNG channel from retinal rods== |
| - | <StructureSection load='7o4h' size='340' side='right'caption='[[7o4h]]' scene=''> | + | <StructureSection load='7o4h' size='340' side='right'caption='[[7o4h]], [[Resolution|resolution]] 3.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7o4h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O4H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O4H FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o4h OCA], [https://pdbe.org/7o4h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o4h RCSB], [https://www.ebi.ac.uk/pdbsum/7o4h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o4h ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o4h OCA], [https://pdbe.org/7o4h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o4h RCSB], [https://www.ebi.ac.uk/pdbsum/7o4h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o4h ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CNGA1_BOVIN CNGA1_BOVIN] Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cGMP which leads to an opening of the cation channel and thereby causing a depolarization of rod photoreceptors. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In rod photoreceptors of the retina, the cyclic nucleotide-gated (CNG) channel is composed of three CNGA and one CNGB subunits, and it closes in response to light activation to generate an electrical signal that is conveyed to the brain. Here we report the cryo-EM structure of the closed state of the native rod CNG channel isolated from bovine retina. The structure reveals differences between CNGA1 and CNGB1 subunits. Three CNGA1 subunits are tethered at their C terminus by a coiled-coil region. The C-helix in the cyclic nucleotide-binding domain of CNGB1 features a different orientation from that in the three CNGA1 subunits. The arginine residue R994 of CNGB1 reaches into the ionic pathway and blocks the pore, thus introducing an additional gate, which is different from the central hydrophobic gate known from homomeric CNGA channels. These results address the long-standing question of how CNGB1 subunits contribute to the function of CNG channels in visual and olfactory neurons. | ||
| + | |||
| + | The structure of the native CNGA1/CNGB1 CNG channel from bovine retinal rods.,Barret DCA, Schertler GFX, Kaupp UB, Marino J Nat Struct Mol Biol. 2022 Jan;29(1):32-39. doi: 10.1038/s41594-021-00700-8. Epub , 2021 Dec 30. PMID:34969975<ref>PMID:34969975</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7o4h" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bos taurus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Barret DCA]] |
| + | [[Category: Marino J]] | ||
Current revision
The structure of the native CNGA1/CNGB1 CNG channel from retinal rods
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