8ra0
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of CysF== | |
| + | <StructureSection load='8ra0' size='340' side='right'caption='[[8ra0]], [[Resolution|resolution]] 1.89Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8ra0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kitasatospora_cystarginea Kitasatospora cystarginea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RA0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.89Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CXM:N-CARBOXYMETHIONINE'>CXM</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ra0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ra0 OCA], [https://pdbe.org/8ra0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ra0 RCSB], [https://www.ebi.ac.uk/pdbsum/8ra0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ra0 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A1W6R555_9ACTN A0A1W6R555_9ACTN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nature has evolved biosynthetic pathways to molecules possessing reactive warheads that inspired the development of many therapeutic agents, including penicillin antibiotics. Peptides armed with electrophilic warheads have proven to be particularly effective covalent inhibitors, providing essential antimicrobial, antiviral and anticancer agents. Here we provide a full characterization of the pathways that nature deploys to assemble peptides with beta-lactone warheads, which are potent proteasome inhibitors with promising anticancer activity. Warhead assembly involves a three-step cryptic methylation sequence, which is likely required to reduce unfavorable electrostatic interactions during the sterically demanding beta-lactonization. Amide-bond synthetase and adenosine triphosphate (ATP)-grasp enzymes couple amino acids to the beta-lactone warhead, generating the bioactive peptide products. After reconstituting the entire pathway to beta-lactone peptides in vitro, we go on to deliver a diverse range of analogs through enzymatic cascade reactions. Our approach is more efficient and cleaner than the synthetic methods currently used to produce clinically important warhead-containing peptides. | ||
| - | + | Cryptic enzymatic assembly of peptides armed with beta-lactone warheads.,Xu G, Torri D, Cuesta-Hoyos S, Panda D, Yates LRL, Zallot R, Bian K, Jia D, Iorgu AI, Levy C, Shepherd SA, Micklefield J Nat Chem Biol. 2024 Jul 1. doi: 10.1038/s41589-024-01657-7. PMID:38951647<ref>PMID:38951647</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Levy | + | <div class="pdbe-citations 8ra0" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Kitasatospora cystarginea]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Levy CW]] | ||
Current revision
Crystal structure of CysF
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