8k32
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The complex structure of SLKARI with NADH at 2.12-angstrom resolution== |
| - | + | <StructureSection load='8k32' size='340' side='right'caption='[[8k32]], [[Resolution|resolution]] 2.12Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[8k32]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Syntrophothermus_lipocalidus_DSM_12680 Syntrophothermus lipocalidus DSM 12680]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8K32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8K32 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.12Å</td></tr> | |
| - | [[Category: | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> |
| - | [[Category: Chen | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8k32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8k32 OCA], [https://pdbe.org/8k32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8k32 RCSB], [https://www.ebi.ac.uk/pdbsum/8k32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8k32 ProSAT]</span></td></tr> |
| - | [[Category: Huang | + | </table> |
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/D7CJ24_SYNLT D7CJ24_SYNLT] Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.[HAMAP-Rule:MF_00435] | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Syntrophothermus lipocalidus DSM 12680]] | ||
| + | [[Category: Chen CY]] | ||
| + | [[Category: Huang CH]] | ||
Current revision
The complex structure of SLKARI with NADH at 2.12-angstrom resolution
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