1sw7
From Proteopedia
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'''Triosephosphate isomerase from Gallus gallus, loop 6 mutant K174N, T175S, A176S''' | '''Triosephosphate isomerase from Gallus gallus, loop 6 mutant K174N, T175S, A176S''' | ||
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[[Category: Sun, J.]] | [[Category: Sun, J.]] | ||
[[Category: Wierenga, R K.]] | [[Category: Wierenga, R K.]] | ||
| - | [[Category: | + | [[Category: Flexible loop]] |
| - | [[Category: | + | [[Category: Hinge]] |
| - | [[Category: | + | [[Category: Tim barrel]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:12:37 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:12, 3 May 2008
Triosephosphate isomerase from Gallus gallus, loop 6 mutant K174N, T175S, A176S
Overview
The conformational switch from open to closed of the flexible loop 6 of triosephosphate isomerase (TIM) is essential for the catalytic properties of TIM. Using a directed evolution approach, active variants of chicken TIM with a mutated C-terminal hinge tripeptide of loop 6 have been generated (Sun,J. and Sampson,N.S., Biochemistry, 1999, 38, 11474-11481). In chicken TIM, the wild-type C-terminal hinge tripeptide is KTA. Detailed enzymological characterization of six variants showed that some of these (LWA, NPN, YSL, KTK) have decreased catalytic efficiency, whereas others (KVA, NSS) are essentially identical with wild-type. The structural characterization of these six variants is reported. No significant structural differences compared with the wild-type are found for KVA, NSS and LWA, but substantial structural adaptations are seen for NPN, YSL and KTK. These structural differences can be understood from the buried position of the alanine side chain in the C-hinge position 3 in the open conformation of wild-type loop 6. Replacement of this alanine with a bulky side chain causes the closed conformation to be favored, which correlates with the decreased catalytic efficiency of these variants. The structural context of loop 6 and loop 7 and their sequence conservation in 133 wild-type sequences is also discussed.
About this Structure
1SW7 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Understanding protein lids: structural analysis of active hinge mutants in triosephosphate isomerase., Kursula I, Salin M, Sun J, Norledge BV, Haapalainen AM, Sampson NS, Wierenga RK, Protein Eng Des Sel. 2004 Apr;17(4):375-82. Epub 2004 May 27. PMID:15166315 Page seeded by OCA on Sat May 3 09:12:37 2008
