6n1c
From Proteopedia
(Difference between revisions)
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== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/Q5ZRW2_LEGPH Q5ZRW2_LEGPH] Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.[HAMAP-Rule:MF_00209] | [https://www.uniprot.org/uniprot/Q5ZRW2_LEGPH Q5ZRW2_LEGPH] Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.[HAMAP-Rule:MF_00209] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Inorganic pyrophosphate (PP(i)) is generated as an intermediate or byproduct of many fundamental metabolic pathways, including DNA/RNA synthesis. The intracellular concentration of PP(i) must be regulated as buildup can inhibit many critical cellular processes. Inorganic pyrophosphatases (PPases) hydrolyze PP(i) into two orthophosphates (P(i)), preventing the toxic accumulation of the PP(i) byproduct in cells and making P(i) available for use in biosynthetic pathways. Here, the crystal structure of a family I inorganic pyrophosphatase from Legionella pneumophila is reported at 2.0 A resolution. L. pneumophila PPase (LpPPase) adopts a homohexameric assembly and shares the oligonucleotide/oligosaccharide-binding (OB) beta-barrel core fold common to many other bacterial family I PPases. LpPPase demonstrated hydrolytic activity against a general substrate, with Mg(2+) being the preferred metal cofactor for catalysis. Legionnaires' disease is a severe respiratory infection caused primarily by L. pneumophila, and thus increased characterization of the L. pneumophila proteome is of interest. | ||
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| + | Characterization of a family I inorganic pyrophosphatase from Legionella pneumophila Philadelphia 1.,Moorefield J, Konuk Y, Norman JO, Abendroth J, Edwards TE, Lorimer DD, Mayclin SJ, Staker BL, Craig JK, Barett KF, Barrett LK, Van Voorhis WC, Myler PJ, McLaughlin KJ Acta Crystallogr F Struct Biol Commun. 2023 Oct 1;79(Pt 10):257-266. doi: , 10.1107/S2053230X23008002. Epub 2023 Sep 20. PMID:37728609<ref>PMID:37728609</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6n1c" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Inorganic pyrophosphatase 3D structures|Inorganic pyrophosphatase 3D structures]] | *[[Inorganic pyrophosphatase 3D structures|Inorganic pyrophosphatase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]] | [[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]] | ||
Current revision
Crystal structure of Inorganic pyrophosphatase from Legionella pneumophila Philadelphia 1
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