7ovt
From Proteopedia
(Difference between revisions)
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==major seeded in vitro fibril morphology from murine SAA1.1 protein== | ==major seeded in vitro fibril morphology from murine SAA1.1 protein== | ||
| - | <StructureSection load='7ovt' size='340' side='right'caption='[[7ovt]]' scene=''> | + | <StructureSection load='7ovt' size='340' side='right'caption='[[7ovt]], [[Resolution|resolution]] 2.69Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OVT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OVT FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OVT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OVT FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ovt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ovt OCA], [https://pdbe.org/7ovt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ovt RCSB], [https://www.ebi.ac.uk/pdbsum/7ovt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ovt ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.69Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ovt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ovt OCA], [https://pdbe.org/7ovt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ovt RCSB], [https://www.ebi.ac.uk/pdbsum/7ovt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ovt ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Several studies showed that seeding of solutions of monomeric fibril proteins with ex vivo amyloid fibrils accelerated the kinetics of fibril formation in vitro but did not necessarily replicate the seed structure. In this research we use cryo-electron microscopy and other methods to analyze the ability of serum amyloid A (SAA)1.1-derived amyloid fibrils, purified from systemic AA amyloidosis tissue, to seed solutions of recombinant SAA1.1 protein. We show that 98% of the seeded fibrils remodel the full fibril structure of the main ex vivo fibril morphology, which we used for seeding, while they are notably different from unseeded in vitro fibrils. The seeded fibrils show a similar proteinase K resistance as ex vivo fibrils and are substantially more stable to proteolytic digestion than unseeded in vitro fibrils. Our data support the view that the fibril morphology contributes to determining proteolytic stability and that pathogenic amyloid fibrils arise from proteolytic selection. | ||
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| + | Cryo-EM demonstrates the in vitro proliferation of an ex vivo amyloid fibril morphology by seeding.,Heerde T, Rennegarbe M, Biedermann A, Savran D, Pfeiffer PB, Hitzenberger M, Baur J, Puscalau-Girtu I, Zacharias M, Schwierz N, Haupt C, Schmidt M, Fandrich M Nat Commun. 2022 Jan 10;13(1):85. doi: 10.1038/s41467-021-27688-5. PMID:35013242<ref>PMID:35013242</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ovt" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
major seeded in vitro fibril morphology from murine SAA1.1 protein
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