7z0z
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7z0z]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z0Z FirstGlance]. <br> | <table><tr><td colspan='2'>[[7z0z]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z0Z FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z0z OCA], [https://pdbe.org/7z0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z0z RCSB], [https://www.ebi.ac.uk/pdbsum/7z0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z0z ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.68Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z0z OCA], [https://pdbe.org/7z0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z0z RCSB], [https://www.ebi.ac.uk/pdbsum/7z0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z0z ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/Q48614_9LACT Q48614_9LACT] | |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Abortive infection (Abi) is a bacterial antiphage defense strategy involving suicide of the infected cell. Some Abi pathways involve polymerases that are related to reverse transcriptases. They are unique in the way they combine the ability to synthesize DNA in a template-independent manner with protein priming. Here, we report crystal and cryo-electron microscopy structures of two Abi polymerases: AbiK and Abi-P2. Both proteins adopt a bilobal structure with an RT-like domain that comprises palm and fingers subdomains and a unique helical domain. AbiK and Abi-P2 adopt a hexameric and trimeric configuration, respectively, which is unprecedented for reverse transcriptases. Biochemical experiments showed that the formation of these oligomers is required for the DNA polymerization activity. The structure of the AbiK-DNA covalent adduct visualized interactions between the 3' end of DNA and the active site and covalent attachment of the 5' end of DNA to a tyrosine residue used for protein priming. Our data reveal a structural basis of the mechanism of highly unusual template-independent protein-priming polymerases. | ||
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| + | Mechanism of protein-primed template-independent DNA synthesis by Abi polymerases.,Figiel M, Gapinska M, Czarnocki-Cieciura M, Zajko W, Sroka M, Skowronek K, Nowotny M Nucleic Acids Res. 2022 Sep 23;50(17):10026-10040. doi: 10.1093/nar/gkac772. PMID:36107766<ref>PMID:36107766</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7z0z" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Abortive infection DNA polymerase AbiK from Lactococcus lactis, Y44F variant
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