8u2b
From Proteopedia
(Difference between revisions)
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==Cryo-EM structure of C.crescentus bNY30a pilus complex== | ==Cryo-EM structure of C.crescentus bNY30a pilus complex== | ||
| - | <StructureSection load='8u2b' size='340' side='right'caption='[[8u2b]], [[Resolution|resolution]] | + | <StructureSection load='8u2b' size='340' side='right'caption='[[8u2b]], [[Resolution|resolution]] 2.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[8u2b]] is a 37 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides Caulobacter vibrioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8U2B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8U2B FirstGlance]. <br> | <table><tr><td colspan='2'>[[8u2b]] is a 37 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides Caulobacter vibrioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8U2B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8U2B FirstGlance]. <br> | ||
| - | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8u2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8u2b OCA], [https://pdbe.org/8u2b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8u2b RCSB], [https://www.ebi.ac.uk/pdbsum/8u2b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8u2b ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8u2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8u2b OCA], [https://pdbe.org/8u2b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8u2b RCSB], [https://www.ebi.ac.uk/pdbsum/8u2b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8u2b ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/A0A290MFS9_CAUVI A0A290MFS9_CAUVI] | [https://www.uniprot.org/uniprot/A0A290MFS9_CAUVI A0A290MFS9_CAUVI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Caulobacter crescentus Tad (tight adherence) pili, part of the type IV pili family, are crucial for mechanosensing, surface adherence, bacteriophage (phage) adsorption, and cell-cycle regulation. Unlike other type IV pilins, Tad pilins lack the typical globular beta sheet domain responsible for pilus assembly and phage binding. The mechanisms of Tad pilus assembly and its interaction with phage PhiCb5 have been elusive. Using cryo-electron microscopy, we unveiled the Tad pilus assembly mechanism, featuring a unique network of hydrogen bonds at its core. We then identified the Tad pilus binding to the PhiCb5 maturation protein (Mat) through its beta region. Notably, the amino terminus of PhiCb5 Mat is exposed outside the capsid and phage/pilus interface, enabling the attachment of fluorescent and affinity tags. These engineered PhiCb5 virions can be efficiently assembled and purified in Escherichia coli, maintaining infectivity against C. crescentus, which presents promising applications, including RNA delivery and phage display. | ||
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| + | Structural mechanisms of Tad pilus assembly and its interaction with an RNA virus.,Wang Y, Theodore M, Xing Z, Narsaria U, Yu Z, Zeng L, Zhang J Sci Adv. 2024 May 3;10(18):eadl4450. doi: 10.1126/sciadv.adl4450. Epub 2024 May , 3. PMID:38701202<ref>PMID:38701202</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 8u2b" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Cryo-EM structure of C.crescentus bNY30a pilus complex
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