1sxj

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[[Image:1sxj.gif|left|200px]]
[[Image:1sxj.gif|left|200px]]
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{{Structure
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|PDB= 1sxj |SIZE=350|CAPTION= <scene name='initialview01'>1sxj</scene>, resolution 2.85&Aring;
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The line below this paragraph, containing "STRUCTURE_1sxj", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATG:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>ATG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE= RFC1, CDC44, YOR217W, YOR50-7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), RFC4, YOL094C, O0923 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), RFC3, YNL290W, N0533 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), RFC2, YJR068W, J1808 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), RFC5, YBR087W, YBR0810 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), POL30, YBR088C, YBR0811 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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|DOMAIN=
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{{STRUCTURE_1sxj| PDB=1sxj | SCENE= }}
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|RELATEDENTRY=[[1plq|1PLQ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxj OCA], [http://www.ebi.ac.uk/pdbsum/1sxj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sxj RCSB]</span>
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}}
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'''Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA)'''
'''Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA)'''
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[[Category: Donnell, M O.]]
[[Category: Donnell, M O.]]
[[Category: Kuriyan, J.]]
[[Category: Kuriyan, J.]]
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[[Category: aaa+ atpase]]
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[[Category: Aaa+ atpase]]
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[[Category: clamp loader]]
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[[Category: Clamp loader]]
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[[Category: dna polymerase]]
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[[Category: Dna polymerase]]
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[[Category: dna sliding clamp]]
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[[Category: Dna sliding clamp]]
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[[Category: dna-binding protein]]
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[[Category: Dna-binding protein]]
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[[Category: processivity clamp]]
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[[Category: Processivity clamp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:15:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:48:18 2008''
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Revision as of 06:15, 3 May 2008

Image:1sxj.gif

Template:STRUCTURE 1sxj

Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA)


Overview

Sliding clamps are ring-shaped proteins that encircle DNA and confer high processivity on DNA polymerases. Here we report the crystal structure of the five-protein clamp loader complex (replication factor-C, RFC) of the yeast Saccharomyces cerevisiae, bound to the sliding clamp (proliferating cell nuclear antigen, PCNA). Tight interfacial coordination of the ATP analogue ATP-gammaS by RFC results in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring. Placement of a model for primed DNA within the central hole of PCNA reveals a striking correspondence between the RFC spiral and the grooves of the DNA double helix. This model, in which the clamp loader complex locks onto primed DNA in a screw-cap-like arrangement, provides a simple explanation for the process by which the engagement of primer-template junctions by the RFC:PCNA complex results in ATP hydrolysis and release of the sliding clamp on DNA.

About this Structure

1SXJ is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex., Bowman GD, O'Donnell M, Kuriyan J, Nature. 2004 Jun 17;429(6993):724-30. PMID:15201901 Page seeded by OCA on Sat May 3 09:15:05 2008

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