8c9c

From Proteopedia

(Difference between revisions)

Current revision

Cryo-EM captures early ribosome assembly in action

Structural highlights

8c9c is a 4 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 6.62Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RL22_ECOLI This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.[HAMAP-Rule:MF_01331_B] The globular domain of the protein is one of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that penetrates into the center of the 70S ribosome where it lines the wall of the exit tunnel. Removal of most of this hairpin (residues 85-95) does not prevent its incorporation into 70S ribosomes. Two of the hairpin residues (91 and 93) seem to be involved in translation elongation arrest of the SecM protein, as their replacement by larger amino acids alleviates the arrest.[HAMAP-Rule:MF_01331_B]

Publication Abstract from PubMed

Ribosome biogenesis is a fundamental multi-step cellular process in all domains of life that involves the production, processing, folding, and modification of ribosomal RNAs (rRNAs) and ribosomal proteins. To obtain insights into the still unexplored early assembly phase of the bacterial 50S subunit, we exploited a minimal in vitro reconstitution system using purified ribosomal components and scalable reaction conditions. Time-limited assembly assays combined with cryo-EM analysis visualizes the structurally complex assembly pathway starting with a particle consisting of ordered density for only ~500 nucleotides of 23S rRNA domain I and three ribosomal proteins. In addition, our structural analysis reveals that early 50S assembly occurs in a domain-wise fashion, while late 50S assembly proceeds incrementally. Furthermore, we find that both ribosomal proteins and folded rRNA helices, occupying surface exposed regions on pre-50S particles, induce, or stabilize rRNA folds within adjacent regions, thereby creating cooperativity.

Cryo-EM captures early ribosome assembly in action.,Qin B, Lauer SM, Balke A, Vieira-Vieira CH, Burger J, Mielke T, Selbach M, Scheerer P, Spahn CMT, Nikolay R Nat Commun. 2023 Feb 17;14(1):898. doi: 10.1038/s41467-023-36607-9. PMID:36797249^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Qin B, Lauer SM, Balke A, Vieira-Vieira CH, Bürger J, Mielke T, Selbach M, Scheerer P, Spahn CMT, Nikolay R. Cryo-EM captures early ribosome assembly in action. Nat Commun. 2023 Feb 17;14(1):898. PMID:36797249 doi:10.1038/s41467-023-36607-9

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/8c9c"

Categories: Escherichia coli | Large Structures | Lauer S | Nikolay R | Qin B

@@ Line 1: / Line 1: @@
-==Time limited cryo-EM reveals early ribosome assembly in action==
+==Cryo-EM captures early ribosome assembly in action==
 <StructureSection load='8c9c' size='340' side='right'caption='[[8c9c]], [[Resolution|resolution]] 6.62&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[8c9c]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C9C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C9C FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c9c OCA], [https://pdbe.org/8c9c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c9c RCSB], [https://www.ebi.ac.uk/pdbsum/8c9c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c9c ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.62&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c9c OCA], [https://pdbe.org/8c9c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c9c RCSB], [https://www.ebi.ac.uk/pdbsum/8c9c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c9c ProSAT]</span></td></tr>
 </table>
 == Function ==

8c9c

From Proteopedia

Current revision

Cryo-EM captures early ribosome assembly in action

Structural highlights

Function

Publication Abstract from PubMed

References

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