1szp
From Proteopedia
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'''A Crystal Structure of the Rad51 Filament''' | '''A Crystal Structure of the Rad51 Filament''' | ||
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[[Category: Symington, L S.]] | [[Category: Symington, L S.]] | ||
[[Category: Zhang, Y.]] | [[Category: Zhang, Y.]] | ||
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| - | [[Category: | + | [[Category: Homologous recombination]] |
| - | [[Category: | + | [[Category: Rad51 filament]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:19:20 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:19, 3 May 2008
A Crystal Structure of the Rad51 Filament
Overview
Rad51, the major eukaryotic homologous recombinase, is important for the repair of DNA damage and the maintenance of genomic diversity and stability. The active form of this DNA-dependent ATPase is a helical filament within which the search for homology and strand exchange occurs. Here we present the crystal structure of a Saccharomyces cerevisiae Rad51 filament formed by a gain-of-function mutant. This filament has a longer pitch than that seen in crystals of Rad51's prokaryotic homolog RecA, and places the ATPase site directly at a new interface between protomers. Although the filament exhibits approximate six-fold symmetry, alternate protein-protein interfaces are slightly different, implying that the functional unit of Rad51 within the filament may be a dimer. Additionally, we show that mutation of His352, which lies at this new interface, markedly disrupts DNA binding.
About this Structure
1SZP is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of a Rad51 filament., Conway AB, Lynch TW, Zhang Y, Fortin GS, Fung CW, Symington LS, Rice PA, Nat Struct Mol Biol. 2004 Aug;11(8):791-6. Epub 2004 Jul 4. PMID:15235592 Page seeded by OCA on Sat May 3 09:19:20 2008
