8a3y

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Current revision (06:03, 31 July 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 8a3y is ON HOLD until sometime in the future
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==Structure of mammalian Pol II-DSIF-SPT6-PAF1-TFIIS-hexasome elongation complex==
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<StructureSection load='8a3y' size='340' side='right'caption='[[8a3y]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8a3y]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A3Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A3Y FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a3y OCA], [https://pdbe.org/8a3y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a3y RCSB], [https://www.ebi.ac.uk/pdbsum/8a3y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a3y ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A7M4DUC2_PIG A0A7M4DUC2_PIG] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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During gene transcription, RNA polymerase II (RNA Pol II) passes nucleosomes with the help of various elongation factors. Here, we show that RNA Pol II achieves efficient nucleosome passage when the human elongation factors DSIF, PAF1 complex (PAF), RTF1, SPT6, and TFIIS are present. The cryo-EM structure of an intermediate of the nucleosome passage shows a partially unraveled hexasome that lacks the proximal H2A-H2B dimer and interacts with the RNA Pol II jaw, DSIF, and the CTR9trestle helix. RNA Pol II adopts a backtracked state with the RNA 3' end dislodged from the active site and bound in the RNA Pol II pore. Additional structures and biochemical data show that human TFIIS enters the RNA Pol II pore and stimulates the cleavage of the backtracked RNA and nucleosome passage.
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Authors:
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Structure of a backtracked hexasomal intermediate of nucleosome transcription.,Farnung L, Ochmann M, Garg G, Vos SM, Cramer P Mol Cell. 2022 Sep 1;82(17):3126-3134.e7. doi: 10.1016/j.molcel.2022.06.027. Epub , 2022 Jul 19. PMID:35858621<ref>PMID:35858621</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 8a3y" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Sus scrofa]]
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[[Category: Cramer P]]
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[[Category: Farnung L]]
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[[Category: Garg G]]
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[[Category: Ochmann M]]
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[[Category: Vos SM]]

Current revision

Structure of mammalian Pol II-DSIF-SPT6-PAF1-TFIIS-hexasome elongation complex

PDB ID 8a3y

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