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1t0k
From Proteopedia
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[[Image:1t0k.gif|left|200px]] | [[Image:1t0k.gif|left|200px]] | ||
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'''Joint X-ray and NMR Refinement of Yeast L30e-mRNA complex''' | '''Joint X-ray and NMR Refinement of Yeast L30e-mRNA complex''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1T0K is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure supersedes the now removed PDB entries | + | 1T0K is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure supersedes the now removed PDB entries and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1cn9 1cn9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Chao, J A.]] | [[Category: Chao, J A.]] | ||
[[Category: Williamson, J R.]] | [[Category: Williamson, J R.]] | ||
| - | [[Category: | + | [[Category: Joint nmr and x-ray refinement]] |
| - | [[Category: | + | [[Category: Mbp fusion protein]] |
| - | [[Category: | + | [[Category: Ribosomal protein l30e]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:21:19 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:21, 3 May 2008
Joint X-ray and NMR Refinement of Yeast L30e-mRNA complex
Overview
L30e, a Saccharomyces cervisiae ribosomal protein, regulates its own expression by binding to a purine-rich asymmetric internal loop located in both its pre-mRNA and mature mRNA. A crystal structure of an MBP-L30e fusion protein in complex with an RNA containing the pre-mRNA regulatory site was solved at 3.24 A. Interestingly, the structure of the RNA differed from that observed in a previously determined NMR structure of the complex. Analysis of the NMR data led to the identification of a single imino proton resonance in the internal loop that had been incorrectly assigned and was principally responsible for the erroneous RNA structure. A structure refinement was performed using both the X-ray diffraction data and the NMR-derived distance and angle restraints. The joint NMR and X-ray refinement resulted in improved stereochemistry and lower crystallographic R factors. The RNA internal loop of the MBP-L30e-mRNA complex adopts the canonical K-turn fold.
About this Structure
1T0K is a Protein complex structure of sequences from Escherichia coli and Saccharomyces cerevisiae. This structure supersedes the now removed PDB entries and 1cn9. Full crystallographic information is available from OCA.
Reference
Joint X-ray and NMR refinement of the yeast L30e-mRNA complex., Chao JA, Williamson JR, Structure. 2004 Jul;12(7):1165-76. PMID:15242593 Page seeded by OCA on Sat May 3 09:21:19 2008
