9bor
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==IkappaBzeta ankyrin repeat domain:NF-kappaB p50 homodimer complex at 2.0 Angstrom resolution== | |
| + | <StructureSection load='9bor' size='340' side='right'caption='[[9bor]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9bor]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9BOR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9BOR FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.997Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HT3:(2R,3S)-HEPTANE-1,2,3-TRIOL'>HT3</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9bor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9bor OCA], [https://pdbe.org/9bor PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9bor RCSB], [https://www.ebi.ac.uk/pdbsum/9bor PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9bor ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/IKBZ_HUMAN IKBZ_HUMAN] Involved in regulation of NF-kappa-B transcription factor complexes (PubMed:16513645, PubMed:16622025). Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation (PubMed:16513645). Inhibits DNA-binding of RELA and NFKB1/p50, and of the NF-kappa-B p65-p50 heterodimer and the NF-kappa-B p50-p50 homodimer (PubMed:16513645). Seems also to activate NF-kappa-B-mediated transcription (PubMed:16622025). In vitro, upon association with NFKB1/p50 has transcriptional activation activity and, together with NFKB1/p50 and RELA, is recruited to LCN2 promoters (PubMed:16622025). Promotes transcription of LCN2 and DEFB4 (PubMed:16622025). Is recruited to IL-6 promoters and activates IL-6 but decreases TNF-alpha production in response to LPS (By similarity). Seems to be involved in the induction of inflammatory genes activated through TLR/IL-1 receptor signaling (By similarity). Involved in the induction of T helper 17 cells (Th17) differentiation upon recognition of antigen by T cell antigen receptor (TCR) (By similarity).[UniProtKB:Q9EST8]<ref>PMID:16513645</ref> <ref>PMID:16622025</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | As part of the efforts to understand nuclear IkappaB function in NF-kappaB-dependent gene expression, we report an X-ray crystal structure of the IkappaBzeta ankyrin repeat domain in complex with the dimerization domain of the NF-kappaB p50 homodimer. IkappaBzeta possesses an N-terminal alpha helix that conveys domain folding stability. Affinity and specificity of the complex depend on a small portion of p50 at the nuclear localization signal. The model suggests that only one p50 subunit supports binding with IkappaBzeta, and biochemical experiments confirm that IkappaBzeta associates with DNA-bound NF-kappaB p50:RelA heterodimers. Comparisons of IkappaBzeta:p50 and p50:kappaB DNA complex crystallographic models indicate that structural rearrangement is necessary for ternary complex formation of IkappaBzeta and p50 with DNA. | ||
| - | + | Structural and biochemical analyses of the nuclear IkappaBzeta protein in complex with the NF-kappaB p50 homodimer.,Zhu N, Rogers WE, Heidary DK, Huxford T Genes Dev. 2024 Jul 19;38(11-12):528-535. doi: 10.1101/gad.351892.124. PMID:38960718<ref>PMID:38960718</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9bor" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Huxford T]] | ||
| + | [[Category: Rogers WE]] | ||
| + | [[Category: Zhu N]] | ||
Current revision
IkappaBzeta ankyrin repeat domain:NF-kappaB p50 homodimer complex at 2.0 Angstrom resolution
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