1t2k

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[[Image:1t2k.gif|left|200px]]
[[Image:1t2k.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1t2k |SIZE=350|CAPTION= <scene name='initialview01'>1t2k</scene>, resolution 3.00&Aring;
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The line below this paragraph, containing "STRUCTURE_1t2k", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= IRF3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), JUN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ATF2, CREB2, CREBP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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{{STRUCTURE_1t2k| PDB=1t2k | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2k OCA], [http://www.ebi.ac.uk/pdbsum/1t2k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t2k RCSB]</span>
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}}
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'''Structure Of The DNA Binding Domains Of IRF3, ATF-2 and Jun Bound To DNA'''
'''Structure Of The DNA Binding Domains Of IRF3, ATF-2 and Jun Bound To DNA'''
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[[Category: Maniatis, T.]]
[[Category: Maniatis, T.]]
[[Category: Panne, D.]]
[[Category: Panne, D.]]
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[[Category: protein dna complex]]
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[[Category: Protein dna complex]]
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[[Category: transcription]]
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[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:26:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:50:20 2008''
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Revision as of 06:26, 3 May 2008

Image:1t2k.gif

Template:STRUCTURE 1t2k

Structure Of The DNA Binding Domains Of IRF3, ATF-2 and Jun Bound To DNA


Overview

Transcriptional activation of the interferon-beta (IFN-beta) gene requires assembly of an enhanceosome containing the transcription factors ATF-2/c-Jun, IRF-3/IRF-7, NF-kappaB and HMGI(Y). These factors cooperatively bind a composite DNA site and activate expression of the IFN-beta gene. The 3.0 A crystal structure of the DNA-binding domains of ATF-2/c-Jun and two IRF-3 molecules in a complex with 31 base pairs (bp) of the PRDIV-PRDIII region of the IFN-beta enhancer shows that association of the four proteins with DNA creates a continuous surface for the recognition of 24 bp. The structure, together with in vitro binding studies and protein mutagenesis, shows that protein-protein interactions are not critical for cooperative binding. Instead, cooperativity arises mainly through nucleotide sequence-dependent structural changes in the DNA that allow formation of complementary DNA conformations. Because the binding sites overlap on the enhancer, the unit of recognition is the entire nucleotide sequence, not the individual subsites.

About this Structure

1T2K is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of ATF-2/c-Jun and IRF-3 bound to the interferon-beta enhancer., Panne D, Maniatis T, Harrison SC, EMBO J. 2004 Nov 10;23(22):4384-93. Epub 2004 Oct 28. PMID:15510218 Page seeded by OCA on Sat May 3 09:26:16 2008

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OCA

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