9b1u
From Proteopedia
(Difference between revisions)
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- | '''Unreleased structure''' | ||
- | + | ==Crystal structure of PqqT with PQQ bound== | |
+ | <StructureSection load='9b1u' size='340' side='right'caption='[[9b1u]], [[Resolution|resolution]] 1.46Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[9b1u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylorubrum_extorquens Methylorubrum extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9B1U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9B1U FirstGlance]. <br> | ||
+ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.46Å</td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9b1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9b1u OCA], [https://pdbe.org/9b1u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9b1u RCSB], [https://www.ebi.ac.uk/pdbsum/9b1u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9b1u ProSAT]</span></td></tr> | ||
+ | </table> | ||
+ | == Function == | ||
+ | [https://www.uniprot.org/uniprot/B7KXB5_METC4 B7KXB5_METC4] | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | The 2011 discovery of the first rare earth-dependent enzyme in methylotrophic Methylobacterium extorquens AM1 prompted intensive research toward understanding the unique chemistry at play in these systems. This enzyme, an alcohol dehydrogenase (ADH), features a La(3+) ion closely associated with redox-active coenzyme pyrroloquinoline quinone (PQQ) and is structurally homologous to the Ca(2+)-dependent ADH from the same organism. AM1 also produces a periplasmic PQQ-binding protein, PqqT, which we have now structurally characterized to 1.46-A resolution by X-ray diffraction. This crystal structure reveals a Lys residue hydrogen-bonded to PQQ at the site analogously occupied by a Lewis acidic cation in ADH. Accordingly, we prepared K(142)A- and K(142)D-PqqT variants to assess the relevance of this site toward metal binding. Isothermal titration calorimetry experiments and titrations monitored by UV-Vis absorption and emission spectroscopies support that K(142)D-PqqT binds tightly (K(d) = 0.6 +/- 0.2 muM) to La(3+) in the presence of bound PQQ and produces spectral signatures consistent with those of ADH enzymes. These spectral signatures are not observed for WT- or K(142)A-variants or upon addition of Ca(2+) to PQQ ⸦ K(142)D-PqqT. Addition of benzyl alcohol to La(3+)-bound PQQ ⸦ K(142)D-PqqT (but not Ca(2+)-bound PQQ ⸦ K(142)D-PqqT, or La(3+)-bound PQQ ⸦ WT-PqqT) produces spectroscopic changes associated with PQQ reduction, and chemical trapping experiments reveal the production of benzaldehyde, supporting ADH activity. By creating a metal binding site that mimics native ADH enzymes, we present a rare earth-dependent artificial metalloenzyme primed for future mechanistic, biocatalytic, and biosensing applications. | ||
- | + | Structure-driven development of a biomimetic rare earth artificial metalloprotein.,Thompson PJ, Boggs DG, Wilson CA, Bruchs AT, Velidandla U, Bridwell-Rabb J, Olshansky L Proc Natl Acad Sci U S A. 2024 Aug 13;121(33):e2405836121. doi: , 10.1073/pnas.2405836121. Epub 2024 Aug 8. PMID:39116128<ref>PMID:39116128</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | [[Category: | + | </div> |
+ | <div class="pdbe-citations 9b1u" style="background-color:#fffaf0;"></div> | ||
+ | == References == | ||
+ | <references/> | ||
+ | __TOC__ | ||
+ | </StructureSection> | ||
+ | [[Category: Large Structures]] | ||
+ | [[Category: Methylorubrum extorquens]] | ||
+ | [[Category: Boggs D]] | ||
+ | [[Category: Bridwell-Rabb J]] | ||
+ | [[Category: Bruchs A]] | ||
+ | [[Category: Olshansky L]] | ||
+ | [[Category: Thompson P]] |
Current revision
Crystal structure of PqqT with PQQ bound
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