1t4i

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[[Image:1t4i.gif|left|200px]]
[[Image:1t4i.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1t4i |SIZE=350|CAPTION= <scene name='initialview01'>1t4i</scene>, resolution 2.5&Aring;
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The line below this paragraph, containing "STRUCTURE_1t4i", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1t4i| PDB=1t4i | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t4i OCA], [http://www.ebi.ac.uk/pdbsum/1t4i PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t4i RCSB]</span>
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}}
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'''Crystal Structure of a DNA Decamer Containing a Thymine-dimer'''
'''Crystal Structure of a DNA Decamer Containing a Thymine-dimer'''
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==About this Structure==
==About this Structure==
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1T4I is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T4I OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T4I OCA].
==Reference==
==Reference==
Crystal structure of a DNA decamer containing a cis-syn thymine dimer., Park H, Zhang K, Ren Y, Nadji S, Sinha N, Taylor JS, Kang C, Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):15965-70. Epub 2002 Nov 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12456887 12456887]
Crystal structure of a DNA decamer containing a cis-syn thymine dimer., Park H, Zhang K, Ren Y, Nadji S, Sinha N, Taylor JS, Kang C, Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):15965-70. Epub 2002 Nov 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12456887 12456887]
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[[Category: Protein complex]]
 
[[Category: Kang, C.]]
[[Category: Kang, C.]]
[[Category: Nadji, S.]]
[[Category: Nadji, S.]]
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[[Category: Taylor, J S.]]
[[Category: Taylor, J S.]]
[[Category: Zhang, K.]]
[[Category: Zhang, K.]]
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[[Category: dna decamer]]
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[[Category: Dna decamer]]
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[[Category: thymine dimer]]
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[[Category: Thymine dimer]]
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[[Category: uv-damaged dna]]
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[[Category: Uv-damaged dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:30:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:51:11 2008''
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Revision as of 06:31, 3 May 2008

Image:1t4i.gif

Template:STRUCTURE 1t4i

Crystal Structure of a DNA Decamer Containing a Thymine-dimer


Overview

It is well known that exposure to UV induces DNA damage, which is the first step in mutagenesis and a major cause of skin cancer. Among a variety of photoproducts, cyclobutane-type pyrimidine photodimers (CPD) are the most abundant primary lesion. Despite its biological importance, the precise relationship between the structure and properties of DNA containing CPD has remained to be elucidated. Here, we report the free (unbound) crystal structure of duplex DNA containing a CPD lesion at a resolution of 2.0 A. Our crystal structure shows that the overall helical axis bends approximately 30 degrees toward the major groove and unwinds approximately 9 degrees, in remarkable agreement with some previous theoretical and experimental studies. There are also significant differences in local structure compared with standard B-DNA, including pinching of the minor groove at the 3' side of the CPD lesion, a severe change of the base pair parameter in the 5' side, and serious widening of both minor and major groves both 3' and 5' of the CPD. Overall, the structure of the damaged DNA differs from undamaged DNA to an extent that DNA repair proteins may recognize this conformation, and the various components of the replicational and transcriptional machinery may be interfered with due to the perturbed local and global structure.

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structure of a DNA decamer containing a cis-syn thymine dimer., Park H, Zhang K, Ren Y, Nadji S, Sinha N, Taylor JS, Kang C, Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):15965-70. Epub 2002 Nov 27. PMID:12456887 Page seeded by OCA on Sat May 3 09:30:59 2008

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