1t5m

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[[Image:1t5m.gif|left|200px]]
[[Image:1t5m.gif|left|200px]]
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{{Structure
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|PDB= 1t5m |SIZE=350|CAPTION= <scene name='initialview01'>1t5m</scene>
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The line below this paragraph, containing "STRUCTURE_1t5m", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=DKA:DECANOIC+ACID'>DKA</scene>, <scene name='pdbligand=DSG:D-ASPARAGINE'>DSG</scene>, <scene name='pdbligand=DSN:D-SERINE'>DSN</scene>, <scene name='pdbligand=KYN:KYNURENINE'>KYN</scene>, <scene name='pdbligand=MEG:(2S,3R)-3-METHYL-GLUTAMIC+ACID'>MEG</scene>, <scene name='pdbligand=ORN:ORNITHINE'>ORN</scene>
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|ACTIVITY=
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1t5m| PDB=1t5m | SCENE= }}
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|RELATEDENTRY=[[1t5n|1T5N]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t5m OCA], [http://www.ebi.ac.uk/pdbsum/1t5m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t5m RCSB]</span>
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'''Structural transitions as determinants of the action of the calcium-dependent antibiotic daptomycin'''
'''Structural transitions as determinants of the action of the calcium-dependent antibiotic daptomycin'''
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==About this Structure==
==About this Structure==
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1T5M is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T5M OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T5M OCA].
==Reference==
==Reference==
Structural transitions as determinants of the action of the calcium-dependent antibiotic daptomycin., Jung D, Rozek A, Okon M, Hancock RE, Chem Biol. 2004 Jul;11(7):949-57. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15271353 15271353]
Structural transitions as determinants of the action of the calcium-dependent antibiotic daptomycin., Jung D, Rozek A, Okon M, Hancock RE, Chem Biol. 2004 Jul;11(7):949-57. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15271353 15271353]
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[[Category: Protein complex]]
 
[[Category: Hancock, R E.]]
[[Category: Hancock, R E.]]
[[Category: Jung, D.]]
[[Category: Jung, D.]]
[[Category: Okon, M.]]
[[Category: Okon, M.]]
[[Category: Rozek, A.]]
[[Category: Rozek, A.]]
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[[Category: antibiotic]]
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[[Category: Antibiotic]]
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[[Category: calcium-dependent]]
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[[Category: Calcium-dependent]]
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[[Category: cubicin]]
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[[Category: Cubicin]]
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[[Category: cyclic lipopeptide]]
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[[Category: Cyclic lipopeptide]]
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[[Category: daptomycin]]
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[[Category: Daptomycin]]
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[[Category: drug]]
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[[Category: Drug]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:33:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:51:36 2008''
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Revision as of 06:33, 3 May 2008

Template:STRUCTURE 1t5m

Structural transitions as determinants of the action of the calcium-dependent antibiotic daptomycin


Overview

Daptomycin is a cyclic anionic lipopeptide antibiotic recently approved for the treatment of complicated skin infections (Cubicin). Its function is dependent on calcium (as Ca2+). Circular dichroism spectroscopy indicated that daptomycin experienced two structural transitions: a transition upon interaction of daptomycin with Ca2+, and a further transition upon interaction with Ca2+ and the bacterial acidic phospholipid, phosphatidyl glycerol. The Ca2+-dependent insertion of daptomycin into model membranes promoted mild and more pronounced perturbations as assessed by the increase of lipid flip-flop and membrane leakage, respectively. The NMR structure of daptomycin indicated that Ca2+ induced a conformational change in daptomycin that increased its amphipathicity. These results are consistent with the hypothesis that the association of Ca2+ with daptomycin permits it to interact with bacterial membranes with effects that are similar to those of the cationic antimicrobial peptides.

About this Structure

Full crystallographic information is available from OCA.

Reference

Structural transitions as determinants of the action of the calcium-dependent antibiotic daptomycin., Jung D, Rozek A, Okon M, Hancock RE, Chem Biol. 2004 Jul;11(7):949-57. PMID:15271353 Page seeded by OCA on Sat May 3 09:33:19 2008

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