8vi8
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Engineered glutamine binding protein and a cobaloxime ligand - no GLN bound== | |
| + | <StructureSection load='8vi8' size='340' side='right'caption='[[8vi8]], [[Resolution|resolution]] 3.21Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8vi8]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8VI8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8VI8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.21Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8vi8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8vi8 OCA], [https://pdbe.org/8vi8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8vi8 RCSB], [https://www.ebi.ac.uk/pdbsum/8vi8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8vi8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/C3THM2_ECOLX C3THM2_ECOLX] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hydrogen-bonding (H-bonding) interactions in metalloprotein active sites can critically regulate enzyme function. Changes in the protein structure triggered by interplay with substrates, products, and partner proteins are often translated to the metallocofactor by way of specific changes in H-bond networks connected to the active site. However, the complexities of metalloprotein architecture and mechanism often preclude our ability to define the precise molecular interactions giving rise to these intricate regulatory pathways. To address this shortcoming, we have developed conformationally switchable artificial metalloproteins (swArMs) in which allosteric Gln-binding triggers protein conformational changes that impact the microenvironment surrounding an installed metallocofactor. Herein, we report a combined structural, spectroscopic, and computational approach to enhance the conformation-dependent changes in H-bond interactions surrounding the metallocofactor site of a swArM. Structure-informed molecular dynamics simulations were employed to predict point mutations that could enhance active site H-bond interactions preferentially in the Gln-bound holo-conformation of the swArM. Testing our predictions via the unique infrared spectral signals associated with the metallocofactor site, we have identified three key residues capable of imparting conformational control over the metallocofactor microenvironment. The resultant swArMs not only model biologically relevant structural regulation but also provide an enhanced Gln-responsive biological probe to be leveraged in future biosensing applications. | ||
| - | + | Conformation-Dependent Hydrogen-Bonding Interactions in a Switchable Artificial Metalloprotein.,Fatima S, Mehrafrooz B, Boggs DG, Ali N, Singh S, Thielges MC, Bridwell-Rabb J, Aksimentiev A, Olshansky L Biochemistry. 2024 Aug 20;63(16):2040-2050. doi: 10.1021/acs.biochem.4c00209. , Epub 2024 Aug 1. PMID:39088332<ref>PMID:39088332</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8vi8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia coli]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Boggs DG]] | ||
| + | [[Category: Bridwell-Rabb J]] | ||
| + | [[Category: Fatima S]] | ||
| + | [[Category: Olshansky L]] | ||
Current revision
Engineered glutamine binding protein and a cobaloxime ligand - no GLN bound
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