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1t7d

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[[Image:1t7d.gif|left|200px]]
[[Image:1t7d.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1t7d |SIZE=350|CAPTION= <scene name='initialview01'>1t7d</scene>, resolution 2.47&Aring;
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The line below this paragraph, containing "STRUCTURE_1t7d", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ARY:ARYLOMYCIN+A2'>ARY</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Signal_peptidase_I Signal peptidase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.89 3.4.21.89] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= LEPB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1t7d| PDB=1t7d | SCENE= }}
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|RELATEDENTRY=[[1b12|1B12]], [[1kn9|1KN9]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7d OCA], [http://www.ebi.ac.uk/pdbsum/1t7d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t7d RCSB]</span>
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}}
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'''Crystal structure of Escherichia coli type I signal peptidase in complex with a lipopeptide inhibitor'''
'''Crystal structure of Escherichia coli type I signal peptidase in complex with a lipopeptide inhibitor'''
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[[Category: Paetzel, M.]]
[[Category: Paetzel, M.]]
[[Category: Page, M G.P.]]
[[Category: Page, M G.P.]]
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[[Category: antibiotic]]
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[[Category: Antibiotic]]
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[[Category: leader peptidase]]
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[[Category: Leader peptidase]]
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[[Category: leader peptide]]
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[[Category: Leader peptide]]
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[[Category: lysine general base]]
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[[Category: Lysine general base]]
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[[Category: non-covalently bound inhibitor]]
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[[Category: Non-covalently bound inhibitor]]
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[[Category: non-ribosomal peptide synthesis]]
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[[Category: Non-ribosomal peptide synthesis]]
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[[Category: peptide]]
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[[Category: Peptide]]
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[[Category: secondary metabolite]]
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[[Category: Secondary metabolite]]
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[[Category: ser/lys dyad]]
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[[Category: Ser/lys dyad]]
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[[Category: signal peptidase]]
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[[Category: Signal peptidase]]
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[[Category: signal peptide]]
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[[Category: Signal peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:37:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:52:26 2008''
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Revision as of 06:37, 3 May 2008

Image:1t7d.gif

Template:STRUCTURE 1t7d

Crystal structure of Escherichia coli type I signal peptidase in complex with a lipopeptide inhibitor


Overview

We report here the crystallographic and biophysical analysis of a soluble, catalytically active fragment of the Escherichia coli type I signal peptidase (SPase Delta2-75) in complex with arylomycin A2. The 2.5-A resolution structure revealed that the inhibitor is positioned with its COOH-terminal carboxylate oxygen (O45) within hydrogen bonding distance of all the functional groups in the catalytic center of the enzyme (Ser90 O-gamma, Lys145 N-zeta, and Ser88 O-gamma) and that it makes beta-sheet type interactions with the beta-strands that line each side of the binding site. Ligand binding studies, calorimetry, fluorescence spectroscopy, and stopped-flow kinetics were also used to analyze the binding mode of this unique non-covalently bound inhibitor. The crystal structure was solved in the space group P4(3)2(1)2. A detailed comparison is made to the previously published acyl-enzyme inhibitor complex structure (space group: P2(1)2(1)2) and the apo-enzyme structure (space group: P4(1)2(1)2). Together this work provides insights into the binding of pre-protein substrates to signal peptidase and will prove helpful in the development of novel antibiotics.

About this Structure

1T7D is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystallographic and biophysical analysis of a bacterial signal peptidase in complex with a lipopeptide-based inhibitor., Paetzel M, Goodall JJ, Kania M, Dalbey RE, Page MG, J Biol Chem. 2004 Jul 16;279(29):30781-90. Epub 2004 May 10. PMID:15136583 Page seeded by OCA on Sat May 3 09:37:36 2008

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