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1t7p

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[[Image:1t7p.gif|left|200px]]
[[Image:1t7p.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1t7p |SIZE=350|CAPTION= <scene name='initialview01'>1t7p</scene>, resolution 2.2&Aring;
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The line below this paragraph, containing "STRUCTURE_1t7p", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=2DA:2&#39;,3&#39;-DIDEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>2DA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DG3:2&#39;-3&#39;-DIDEOXYGUANOSINE-5&#39;-TRIPHOSPHATE'>DG3</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1t7p| PDB=1t7p | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7p OCA], [http://www.ebi.ac.uk/pdbsum/1t7p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t7p RCSB]</span>
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}}
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'''T7 DNA POLYMERASE COMPLEXED TO DNA PRIMER/TEMPLATE,A NUCLEOSIDE TRIPHOSPHATE, AND ITS PROCESSIVITY FACTOR THIOREDOXIN'''
'''T7 DNA POLYMERASE COMPLEXED TO DNA PRIMER/TEMPLATE,A NUCLEOSIDE TRIPHOSPHATE, AND ITS PROCESSIVITY FACTOR THIOREDOXIN'''
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[[Category: Richardson, C C.]]
[[Category: Richardson, C C.]]
[[Category: Tabor, S.]]
[[Category: Tabor, S.]]
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[[Category: complex (hydrolase/electron transport/dna)]]
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[[Category: Dna replication]]
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[[Category: dna replication]]
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[[Category: Nucleotidyl transferase]]
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[[Category: nucleotidyl transferase]]
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[[Category: Processivity factor]]
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[[Category: processivity factor]]
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[[Category: Sequencing]]
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[[Category: sequencing]]
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[[Category: T7 dna polymerase]]
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[[Category: t7 dna polymerase]]
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[[Category: Thioredoxin]]
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[[Category: thioredoxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:38:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:52:26 2008''
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Revision as of 06:38, 3 May 2008

Image:1t7p.gif

Template:STRUCTURE 1t7p

T7 DNA POLYMERASE COMPLEXED TO DNA PRIMER/TEMPLATE,A NUCLEOSIDE TRIPHOSPHATE, AND ITS PROCESSIVITY FACTOR THIOREDOXIN


Overview

DNA polymerases change their specificity for nucleotide substrates with each catalytic cycle, while achieving error frequencies in the range of 10(-5) to 10(-6). Here we present a 2.2 A crystal structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer-template and a nucleoside triphosphate in the polymerase active site. The structure illustrates how nucleotides are selected in a template-directed manner, and provides a structural basis for a metal-assisted mechanism of phosphoryl transfer by a large group of related polymerases.

About this Structure

1T7P is a Protein complex structure of sequences from Enterobacteria phage t7 and Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution., Doublie S, Tabor S, Long AM, Richardson CC, Ellenberger T, Nature. 1998 Jan 15;391(6664):251-8. PMID:9440688 Page seeded by OCA on Sat May 3 09:38:21 2008

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