8v2v
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution NMR structure of recifin A [Y6F]== | |
| + | <StructureSection load='8v2v' size='340' side='right'caption='[[8v2v]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8v2v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Axinella_sp._1_TF-2017 Axinella sp. 1 TF-2017]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8V2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8V2V FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8v2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8v2v OCA], [https://pdbe.org/8v2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8v2v RCSB], [https://www.ebi.ac.uk/pdbsum/8v2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8v2v ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The peptide recifin A is the inaugural member of the structurally intriguing new fold referred to as a tyrosine-lock. Its central four stranded beta-sheet is stabilized by a unique arrangement in which three disulfide bonds and their interconnecting backbone form a ring that wraps around one of the strands, resulting in a Tyr side chain being buried in the molecular core. Here we aimed to establish a synthetic route to this complex class of natural products. Full length recifin A was successfully generated through native chemical ligation chemistry joining two 21 amino acid residue fragments. Surprisingly, reduced linear recifin A readily adopts the correct, topologically-complex fold via random oxidation of the cysteines, suggesting it is highly energetically favored. Utilizing our synthetic strategy, we generated five recifin A analogues to investigate the structural role of the central Tyr residue and provide the first insights into the structure activity relationship of recifin A towards its cancer target tyrosyl-DNA phosphodiesterase I. | ||
| - | + | Picking the tyrosine-lock: chemical synthesis of the tyrosyl-DNA phosphodiesterase I inhibitor recifin A and analogues.,Smallwood TB, Krumpe LRH, Payne CD, Klein VG, O'Keefe BR, Clark RJ, Schroeder CI, Rosengren KJ Chem Sci. 2024 Jun 14;15(33):13227-13233. doi: 10.1039/d4sc01976h. eCollection , 2024 Aug 22. PMID:39183914<ref>PMID:39183914</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8v2v" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Axinella sp. 1 TF-2017]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Payne CD]] | ||
| + | [[Category: Rosengren KJ]] | ||
| + | [[Category: Schroeder CI]] | ||
Current revision
Solution NMR structure of recifin A [Y6F]
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