1ta1

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[[Image:1ta1.gif|left|200px]]
[[Image:1ta1.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1ta1 |SIZE=350|CAPTION= <scene name='initialview01'>1ta1</scene>, resolution 2.50&Aring;
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The line below this paragraph, containing "STRUCTURE_1ta1", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Arginase Arginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.1 3.5.3.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= ARG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
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-->
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|DOMAIN=
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{{STRUCTURE_1ta1| PDB=1ta1 | SCENE= }}
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|RELATEDENTRY=[[1rla|1RLA]], [[1tbh|1TBH]], [[1tbj|1TBJ]], [[1tbl|1TBL]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ta1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ta1 OCA], [http://www.ebi.ac.uk/pdbsum/1ta1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ta1 RCSB]</span>
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}}
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'''H141C mutant of rat liver arginase I'''
'''H141C mutant of rat liver arginase I'''
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[[Category: Christianson, D W.]]
[[Category: Christianson, D W.]]
[[Category: Cox, J D.]]
[[Category: Cox, J D.]]
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[[Category: arginase]]
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[[Category: Arginase]]
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[[Category: binuclear manganese cluster]]
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[[Category: Binuclear manganese cluster]]
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[[Category: h141c mutation]]
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[[Category: H141c mutation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:43:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:53:24 2008''
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Revision as of 06:43, 3 May 2008

Image:1ta1.gif

Template:STRUCTURE 1ta1

H141C mutant of rat liver arginase I


Overview

Rat liver arginase (arginase I) is potently inactivated by diethyl pyrocarbonate, with a second-order rate constant of 113M(-1)s(-1) for the inactivation process at pH 7.0, 25 degrees C. Partial protection from inactivation is provided by the product of the reaction, l-ornithine, while nearly complete protection is afforded by the inhibitor pair, l-ornithine and borate. The role of H141 has been probed by mutagenesis, chemical modulation, and X-ray diffraction. The hyper-reactivity of H141 towards diethyl pyrocarbonate can be explained by its proximity to E277. A proton shuttling role for H141 is supported by its conformational mobility observed among the known arginase structures. H141 is proposed to serve as an acid/base catalyst, deprotonating the metal-bridging water molecule to generate the metal-bridging hydroxide nucleophile, and by protonating the amino group of the product to facilitate its departure.

About this Structure

1TA1 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Probing the role of the hyper-reactive histidine residue of arginase., Colleluori DM, Reczkowski RS, Emig FA, Cama E, Cox JD, Scolnick LR, Compher K, Jude K, Han S, Viola RE, Christianson DW, Ash DE, Arch Biochem Biophys. 2005 Dec 1;444(1):15-26. Epub 2005 Oct 13. PMID:16266687 Page seeded by OCA on Sat May 3 09:43:37 2008

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