1tad

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[[Image:1tad.gif|left|200px]]
[[Image:1tad.gif|left|200px]]
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{{Structure
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|PDB= 1tad |SIZE=350|CAPTION= <scene name='initialview01'>1tad</scene>, resolution 1.7&Aring;
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The line below this paragraph, containing "STRUCTURE_1tad", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene>
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{{STRUCTURE_1tad| PDB=1tad | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tad FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tad OCA], [http://www.ebi.ac.uk/pdbsum/1tad PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tad RCSB]</span>
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'''GTPASE MECHANISM OF GPROTEINS FROM THE 1.7-ANGSTROM CRYSTAL STRUCTURE OF TRANSDUCIN ALPHA-GDP-ALF4-'''
'''GTPASE MECHANISM OF GPROTEINS FROM THE 1.7-ANGSTROM CRYSTAL STRUCTURE OF TRANSDUCIN ALPHA-GDP-ALF4-'''
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[[Category: Sigler, P B.]]
[[Category: Sigler, P B.]]
[[Category: Sondek, J.]]
[[Category: Sondek, J.]]
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[[Category: gtp-binding protein]]
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[[Category: Gtp-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:44:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:53:31 2008''
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Revision as of 06:44, 3 May 2008

Image:1tad.gif

Template:STRUCTURE 1tad

GTPASE MECHANISM OF GPROTEINS FROM THE 1.7-ANGSTROM CRYSTAL STRUCTURE OF TRANSDUCIN ALPHA-GDP-ALF4-


Overview

Aluminium fluoride (AIF-4) activates members of the heterotrimeric G-protein (G alpha beta gamma) family by binding to inactive G alpha.GDP near the site occupied by the gamma-phosphate in G alpha.GTP (ref. 3). Here we describe the crystal structure of transducin alpha.GDP activated with aluminium fluoride (Gt alpha.GDP.AIF-4.H2O) at 1.7 A, a resolution sufficient to establish the coordination geometry of the bound aluminium fluoride as well as the extensive network of direct and water-mediated interactions that stabilize it. These observations are derived from three independent representations in the asymmetric unit, eliminating any chance of drawing conclusions based on stereochemistry imposed by crystal packing. Surprisingly, aluminium fluoride activates G alpha.GDP by binding with a geometry resembling a pentavalent intermediate for GTP hydrolysis. The stabilizing interactions involve not only residues that interact with the gamma-phosphate in Gt alpha.GTP gamma S, but also conserved residues for GTPase activity. Thus the Gt alpha.GDP.AIF-4.H2O structure provides new insight into the mechanism of GTP hydrolysis.

About this Structure

1TAD is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

GTPase mechanism of Gproteins from the 1.7-A crystal structure of transducin alpha-GDP-AIF-4., Sondek J, Lambright DG, Noel JP, Hamm HE, Sigler PB, Nature. 1994 Nov 17;372(6503):276-9. PMID:7969474 Page seeded by OCA on Sat May 3 09:44:17 2008

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