| Structural highlights
Function
TTTP_BPT5 Plays an essential role in tail assembly by capping the rapidly polymerizing tail once it has reached its requisite length.[1] [2]
Publication Abstract from PubMed
T5 is a siphophage that has been extensively studied by structural and biochemical methods. However, the complete in situ structures of T5 before and after DNA ejection remain unknown. In this study, we used cryo-electron microscopy (cryo-EM) to determine the structures of mature T5 (a laboratory-adapted, fiberless T5 mutant) and urea-treated empty T5 (lacking the tip complex) at near-atomic resolutions. Atomic models of the head, connector complex, tail tube, and tail tip were built for mature T5, and atomic models of the connector complex, comprising the portal protein pb7, adaptor protein p144, and tail terminator protein p142, were built for urea-treated empty T5. Our findings revealed that the aforementioned proteins did not undergo global conformational changes before and after DNA ejection, indicating that these structural features were conserved among most myophages and siphophages. The present study elucidates the underlying mechanisms of siphophage infection and DNA ejection.
Structures of Mature and Urea-Treated Empty Bacteriophage T5: Insights into Siphophage Infection and DNA Ejection.,Peng Y, Tang H, Xiao H, Chen W, Song J, Zheng J, Liu H Int J Mol Sci. 2024 Aug 3;25(15):8479. doi: 10.3390/ijms25158479. PMID:39126049[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Linares R, Arnaud CA, Effantin G, Darnault C, Epalle NH, Boeri Erba E, Schoehn G, Breyton C. Structural basis of bacteriophage T5 infection trigger and E. coli cell wall perforation. Sci Adv. 2023 Mar 24;9(12):eade9674. PMID:36961893 doi:10.1126/sciadv.ade9674
- ↑ Zivanovic Y, Confalonieri F, Ponchon L, Lurz R, Chami M, Flayhan A, Renouard M, Huet A, Decottignies P, Davidson AR, Breyton C, Boulanger P. Insights into bacteriophage T5 structure from analysis of its morphogenesis genes and protein components. J Virol. 2014 Jan;88(2):1162-74. doi: 10.1128/JVI.02262-13. Epub 2013 Nov 6. PMID:24198424 doi:http://dx.doi.org/10.1128/JVI.02262-13
- ↑ Peng Y, Tang H, Xiao H, Chen W, Song J, Zheng J, Liu H. Structures of Mature and Urea-Treated Empty Bacteriophage T5: Insights into Siphophage Infection and DNA Ejection. Int J Mol Sci. 2024 Aug 3;25(15):8479. PMID:39126049 doi:10.3390/ijms25158479
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