1tc3
From Proteopedia
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[[Image:1tc3.gif|left|200px]] | [[Image:1tc3.gif|left|200px]] | ||
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'''TRANSPOSASE TC3A1-65 FROM CAENORHABDITIS ELEGANS''' | '''TRANSPOSASE TC3A1-65 FROM CAENORHABDITIS ELEGANS''' | ||
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[[Category: Pouderoyen, G Van.]] | [[Category: Pouderoyen, G Van.]] | ||
[[Category: Sixma, T K.]] | [[Category: Sixma, T K.]] | ||
| - | [[Category: | + | [[Category: Dna binding]] |
| - | + | [[Category: Helix-turn-helix]] | |
| - | [[Category: | + | [[Category: Tc1/mariner family]] |
| - | [[Category: | + | [[Category: Transposase]] |
| - | [[Category: | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:47:05 2008'' |
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:47, 3 May 2008
TRANSPOSASE TC3A1-65 FROM CAENORHABDITIS ELEGANS
Overview
The crystal structure of the complex between the N-terminal DNA-binding domain of Tc3 transposase and an oligomer of transposon DNA has been determined. The specific DNA-binding domain contains three alpha-helices, of which two form a helix-turn-helix (HTH) motif. The recognition of transposon DNA by the transposase is mediated through base-specific contacts and complementarity between protein and sequence-dependent deformations of the DNA. The HTH motif makes four base-specific contacts with the major groove, and the N-terminus makes three base-specific contacts with the minor groove. The DNA oligomer adopts a non-linear B-DNA conformation, made possible by a stretch of seven G:C base pairs at one end and a TATA sequence towards the other end. Extensive contacts (seven salt bridges and 16 hydrogen bonds) of the protein with the DNA backbone allow the protein to probe and recognize the sequence-dependent DNA deformation. The DNA-binding domain forms a dimer in the crystals. Each monomer binds a separate transposon end, implying that the dimer plays a role in synapsis, necessary for the simultaneous cleavage of both transposon termini.
About this Structure
1TC3 is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Crystal structure of the specific DNA-binding domain of Tc3 transposase of C.elegans in complex with transposon DNA., van Pouderoyen G, Ketting RF, Perrakis A, Plasterk RH, Sixma TK, EMBO J. 1997 Oct 1;16(19):6044-54. PMID:9312061 Page seeded by OCA on Sat May 3 09:47:05 2008
