3ngg

From Proteopedia

(Difference between revisions)

Current revision

X-ray Structure of Omwaprin

Structural highlights

3ngg is a 2 chain structure with sequence from Oxyuranus microlepidotus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.33Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

WAPA_OXYMI

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The 50-residue snake venom protein L-omwaprin and its enantiomer D-omwaprin were prepared by total chemical synthesis. Radial diffusion assays were performed against Bacillus megaterium and Bacillus anthracis; both L-omwaprin and D-omwaprin showed anti-bacterial activity against B. megaterium. The native protein enantiomer, made of L- amino acids, failed to crystallize readily. However, when a racemic mixture containing equal amounts of L-omwaprin and D-omwaprin was used, diffraction quality crystals were obtained. The racemic protein sample crystallized in the centrosymmetric space group P2(1)/c and its structure was determined at atomic resolution (1.33 A) by a combination of Patterson and direct methods based on the strong scattering from the sulfur atoms in the eight cysteine residues per protein. Racemic crystallography once again proved to be a valuable method for obtaining crystals of recalcitrant proteins, and for determining high resolution X-ray structures by direct methods.

Determination of the X-ray structure of the snake venom protein omwaprin by total chemical synthesis and racemic protein crystallography.,Banigan JR, Mandal K, Sawaya MR, Thammavongsa V, Hendrickx AP, Schneewind O, Yeates TO, H Kent SB Protein Sci. 2010 Jul 28. PMID:20669184^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Banigan JR, Mandal K, Sawaya MR, Thammavongsa V, Hendrickx AP, Schneewind O, Yeates TO, H Kent SB. Determination of the X-ray structure of the snake venom protein omwaprin by total chemical synthesis and racemic protein crystallography. Protein Sci. 2010 Jul 28. PMID:20669184 doi:10.1002/pro.468

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ngg"

@@ Line 3: / Line 3: @@
 <StructureSection load='3ngg' size='340' side='right'caption='[[3ngg]], [[Resolution|resolution]] 1.33&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ngg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NGG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3NGG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ngg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oxyuranus_microlepidotus Oxyuranus microlepidotus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NGG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NGG FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3ngg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ngg OCA], [http://pdbe.org/3ngg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ngg RCSB], [http://www.ebi.ac.uk/pdbsum/3ngg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ngg ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.33&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ngg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ngg OCA], [https://pdbe.org/3ngg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ngg RCSB], [https://www.ebi.ac.uk/pdbsum/3ngg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ngg ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/WAPA_OXYMI WAPA_OXYMI]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 11: / Line 14: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ng/3ngg_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 30: / Line 33: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Banigan, J R]]
+[[Category: Oxyuranus microlepidotus]]
-[[Category: Hendrickx, A P.A]]
+[[Category: Banigan JR]]
-[[Category: Kent, S B.H]]
+[[Category: Hendrickx APA]]
-[[Category: Mandal, K]]
+[[Category: Kent SBH]]
-[[Category: Sawaya, M R]]
+[[Category: Mandal K]]
-[[Category: Schneewind, O]]
+[[Category: Sawaya MR]]
-[[Category: Thammavongsa, V]]
+[[Category: Schneewind O]]
-[[Category: Yeates, T O]]
+[[Category: Thammavongsa V]]
-[[Category: Antibiotic]]
+[[Category: Yeates TO]]
-[[Category: Direct method]]
-[[Category: Racemic protein crystallography]]
-[[Category: Venom protein]]

3ngg

From Proteopedia

Current revision

X-ray Structure of Omwaprin

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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