|
|
| Line 3: |
Line 3: |
| | <StructureSection load='5vem' size='340' side='right'caption='[[5vem]], [[Resolution|resolution]] 2.60Å' scene=''> | | <StructureSection load='5vem' size='340' side='right'caption='[[5vem]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5vem]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VEM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VEM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5vem]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VEM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VEM FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5veo|5veo]], [[5ven|5ven]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ENPP5, UNQ550/PRO1107 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vem OCA], [https://pdbe.org/5vem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vem RCSB], [https://www.ebi.ac.uk/pdbsum/5vem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vem ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vem OCA], [http://pdbe.org/5vem PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vem RCSB], [http://www.ebi.ac.uk/pdbsum/5vem PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vem ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ENPP5_HUMAN ENPP5_HUMAN]] May play a role in neuronal cell communication. Lacks nucleotide pyrophosphatase and lysopholipase D activity (By similarity).[UniProtKB:P84039] | + | [https://www.uniprot.org/uniprot/ENPP5_HUMAN ENPP5_HUMAN] May play a role in neuronal cell communication. Lacks nucleotide pyrophosphatase and lysopholipase D activity (By similarity).[UniProtKB:P84039] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 27: |
Line 26: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Gorelik, A]] | + | [[Category: Gorelik A]] |
| - | [[Category: Illes, K]] | + | [[Category: Illes K]] |
| - | [[Category: Nagar, B]] | + | [[Category: Nagar B]] |
| - | [[Category: Randriamihaja, A]] | + | [[Category: Randriamihaja A]] |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
ENPP5_HUMAN May play a role in neuronal cell communication. Lacks nucleotide pyrophosphatase and lysopholipase D activity (By similarity).[UniProtKB:P84039]
Publication Abstract from PubMed
The ecto-nucleotide pyrophosphatase / phosphodiesterase (NPP) family of proteins mediates purinergic signaling by degrading extracellular nucleotides, and also participates in phospholipid metabolism. NPP5 (ENPP5) is the least characterized member of this group and its specific role is unknown. This enzyme does not display activity on certain nucleotides and on other typical NPP substrates. In order to gain insights into its function, we determined the crystal structure of human and murine NPP5. Structural comparison with close homologs revealed a key phenylalanine to tyrosine substitution that prevents efficient hydrolysis of nucleotide diphosphates and triphosphates; reversal of this mutation enabled degradation of these molecules. Interestingly, NPP5 is able to cleave nicotinamide adenine dinucleotide (NAD), suggesting a potential role of this enzyme in NAD-based neurotransmission. An NPP5-specific metal binding motif is found adjacent to the active site, although its significance is unclear. These findings expand our understanding of substrate specificity within the NPP family. This article is protected by copyright. All rights reserved.
A Key Tyrosine Substitution Restricts Nucleotide Hydrolysis by the Ectoenzyme NPP5.,Gorelik A, Randriamihaja A, Illes K, Nagar B FEBS J. 2017 Sep 12. doi: 10.1111/febs.14266. PMID:28898552[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Gorelik A, Randriamihaja A, Illes K, Nagar B. A Key Tyrosine Substitution Restricts Nucleotide Hydrolysis by the Ectoenzyme NPP5. FEBS J. 2017 Sep 12. doi: 10.1111/febs.14266. PMID:28898552 doi:http://dx.doi.org/10.1111/febs.14266
|
