6jiw

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of S. aureus CntK with C72S mutation

Structural highlights

6jiw is a 1 chain structure with sequence from Staphylococcus aureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.58Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CNTK_STAAM Catalyzes the reversible interconversion of the L- and D-stereoisomers of histidine. Functions in the biosynthesis of the metallophore staphylopine, which is involved in the acquisition of nickel, cobalt, zinc, copper, and iron, and thus enables bacterial growth inside the host, where metal access is limited. Therefore, this enzyme probably contributes to staphylococcal virulence. Appears to be specific for histidine, as it cannot use L-alanine and L-methionine as substrate.^[1]

Publication Abstract from PubMed

Staphylopine is a newly identified broad-spectrum metallophore for metal acquisition, and it plays important roles in the survival and virulence of Staphylococcus aureus and other pathogens in the metal-scarce environment in hosts. CntK catalyzes the first step of staphylopine synthesis by converting L-histidine to D-histidine to provide an essential building block of staphylopine. Herein, the crystal structures of S. aureus CntK (SaCntK) and its C72S variant are determined at 1.82 and 1.58A resolution, respectively. SaCntK forms a homodimer and each subunit contains a two-domain alpha/beta structure. Its overall structure resembles diaminopimelate epimerase, although their sequence identities are lower than 22%. SaCntK is specific for histidine, whereas other proteinogenic amino acids, with the exception of arginine, do not show any binding with SaCntK. Structural modeling suggested that residues Asn16, Glu46, Gln47 and Glu208 are responsible for specific substrate binding, and their substitutions significantly reduced the binding of histidine to SaCntK. Structural modeling suggested SaCntK uses a two-base catalytic mechanism, which has been observed in many cofactor-independent racemases. Our study provides critical insights into the structure and functions of CntK in staphylopine synthesis, which makes it helpful for developing potential antibiotics targeting the staphylopine-mediated metal acquisition process in bacteria.

Crystal structure of CntK, the cofactor-independent histidine racemase in staphylopine-mediated metal acquisition of Staphylococcus aureus.,Luo S, Ju Y, Zhou J, Gu Q, Xu J, Zhou H Int J Biol Macromol. 2019 Aug 15;135:725-733. doi:, 10.1016/j.ijbiomac.2019.05.169. Epub 2019 May 23. PMID:31129210^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ghssein G, Brutesco C, Ouerdane L, Fojcik C, Izaute A, Wang S, Hajjar C, Lobinski R, Lemaire D, Richaud P, Voulhoux R, Espaillat A, Cava F, Pignol D, Borezée-Durant E, Arnoux P. Biosynthesis of a broad-spectrum nicotianamine-like metallophore in Staphylococcus aureus. Science. 2016 May 27;352(6289):1105-9. PMID:27230378 doi:10.1126/science.aaf1018
↑ Luo S, Ju Y, Zhou J, Gu Q, Xu J, Zhou H. Crystal structure of CntK, the cofactor-independent histidine racemase in staphylopine-mediated metal acquisition of Staphylococcus aureus. Int J Biol Macromol. 2019 Aug 15;135:725-733. doi:, 10.1016/j.ijbiomac.2019.05.169. Epub 2019 May 23. PMID:31129210 doi:http://dx.doi.org/10.1016/j.ijbiomac.2019.05.169

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6jiw"

Categories: Large Structures | Staphylococcus aureus | Ju Y | Luo S | Zhou H

@@ Line 3: / Line 3: @@
 <StructureSection load='6jiw' size='340' side='right'caption='[[6jiw]], [[Resolution|resolution]] 1.58&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6jiw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JIW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JIW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6jiw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JIW FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.58&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6jis|6jis]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jiw OCA], [https://pdbe.org/6jiw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jiw RCSB], [https://www.ebi.ac.uk/pdbsum/6jiw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jiw ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jiw OCA], [http://pdbe.org/6jiw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jiw RCSB], [http://www.ebi.ac.uk/pdbsum/6jiw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jiw ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/CNTK_STAAM CNTK_STAAM] Catalyzes the reversible interconversion of the L- and D-stereoisomers of histidine. Functions in the biosynthesis of the metallophore staphylopine, which is involved in the acquisition of nickel, cobalt, zinc, copper, and iron, and thus enables bacterial growth inside the host, where metal access is limited. Therefore, this enzyme probably contributes to staphylococcal virulence. Appears to be specific for histidine, as it cannot use L-alanine and L-methionine as substrate.<ref>PMID:27230378</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Ju, Y]]
+[[Category: Staphylococcus aureus]]
-[[Category: Luo, S]]
+[[Category: Ju Y]]
-[[Category: Zhou, H]]
+[[Category: Luo S]]
-[[Category: Cobalt and nickel transporter system]]
+[[Category: Zhou H]]
-[[Category: Histidine racemase]]
-[[Category: Isomerase]]

6jiw

From Proteopedia

Current revision

Crystal structure of S. aureus CntK with C72S mutation

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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