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| <StructureSection load='6sj9' size='340' side='right'caption='[[6sj9]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='6sj9' size='340' side='right'caption='[[6sj9]], [[Resolution|resolution]] 2.20Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[6sj9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_13344 Atcc 13344]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SJ9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6sj9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenarthrobacter_aurescens Paenarthrobacter aurescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SJ9 FirstGlance]. <br> |
- | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
- | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> |
- | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AAur_2182 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43663 ATCC 13344])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sj9 OCA], [https://pdbe.org/6sj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sj9 RCSB], [https://www.ebi.ac.uk/pdbsum/6sj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sj9 ProSAT]</span></td></tr> |
- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sj9 OCA], [http://pdbe.org/6sj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sj9 RCSB], [http://www.ebi.ac.uk/pdbsum/6sj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sj9 ProSAT]</span></td></tr> | + | |
| </table> | | </table> |
| + | == Function == |
| + | [https://www.uniprot.org/uniprot/A0A4Y3NDN0_PAEAU A0A4Y3NDN0_PAEAU] |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
- | [[Category: Atcc 13344]] | |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
- | [[Category: Ban, N]] | + | [[Category: Paenarthrobacter aurescens]] |
- | [[Category: Leibundgut, M]] | + | [[Category: Ban N]] |
- | [[Category: Mueller, A U]] | + | [[Category: Leibundgut M]] |
- | [[Category: Weber-Ban, E]] | + | [[Category: Mueller AU]] |
- | [[Category: Dna damage response]]
| + | [[Category: Weber-Ban E]] |
- | [[Category: Ferredoxin-like fold]]
| + | |
- | [[Category: Sm-fold]]
| + | |
- | [[Category: Transcription]]
| + | |
- | [[Category: Transcriptional regulator]]
| + | |
- | [[Category: Winged helix-turn-helix]]
| + | |
- | [[Category: Wyl domain]]
| + | |
| Structural highlights
6sj9 is a 2 chain structure with sequence from Paenarthrobacter aurescens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| Method: | X-ray diffraction, Resolution 2.2Å |
Ligands: | , , , , |
Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
A0A4Y3NDN0_PAEAU
Publication Abstract from PubMed
In mycobacteria, transcriptional activator PafBC is responsible for upregulating the majority of genes induced by DNA damage. Understanding the mechanism of PafBC activation is impeded by a lack of structural information on this transcription factor that contains a widespread, but poorly understood WYL domain frequently encountered in bacterial transcription factors. Here, we determine the crystal structure of Arthrobacter aurescens PafBC. The protein consists of two modules, each harboring an N-terminal helix-turn-helix DNA-binding domain followed by a central WYL and a C-terminal extension (WCX) domain. The WYL domains exhibit Sm-folds, while the WCX domains adopt ferredoxin-like folds, both characteristic for RNA-binding proteins. Our results suggest a mechanism of regulation in which WYL domain-containing transcription factors may be activated by binding RNA or other nucleic acid molecules. Using an in vivo mutational screen in Mycobacterium smegmatis, we identify potential co-activator binding sites on PafBC.
Structure and functional implications of WYL domain-containing bacterial DNA damage response regulator PafBC.,Muller AU, Leibundgut M, Ban N, Weber-Ban E Nat Commun. 2019 Oct 11;10(1):4653. doi: 10.1038/s41467-019-12567-x. PMID:31604936[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Muller AU, Leibundgut M, Ban N, Weber-Ban E. Structure and functional implications of WYL domain-containing bacterial DNA damage response regulator PafBC. Nat Commun. 2019 Oct 11;10(1):4653. doi: 10.1038/s41467-019-12567-x. PMID:31604936 doi:http://dx.doi.org/10.1038/s41467-019-12567-x
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