7mjl
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Cryo-EM structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain bound to Fab ab1 (focused refinement of RBD and Fab ab1)== |
| - | <StructureSection load='7mjl' size='340' side='right'caption='[[7mjl]]' scene=''> | + | <StructureSection load='7mjl' size='340' side='right'caption='[[7mjl]], [[Resolution|resolution]] 2.95Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7mjl]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MJL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MJL FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mjl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mjl OCA], [https://pdbe.org/7mjl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mjl RCSB], [https://www.ebi.ac.uk/pdbsum/7mjl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mjl ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.95Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mjl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mjl OCA], [https://pdbe.org/7mjl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mjl RCSB], [https://www.ebi.ac.uk/pdbsum/7mjl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mjl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The recently reported "UK variant" (B.1.1.7) of SARS-CoV-2 is thought to be more infectious than previously circulating strains as a result of several changes, including the N501Y mutation. We present a 2.9-A resolution cryo-electron microscopy (cryo-EM) structure of the complex between the ACE2 receptor and N501Y spike protein ectodomains that shows Y501 inserted into a cavity at the binding interface near Y41 of ACE2. This additional interaction provides a structural explanation for the increased ACE2 affinity of the N501Y mutant, and likely contributes to its increased infectivity. However, this mutation does not result in large structural changes, enabling important neutralization epitopes to be retained in the spike receptor binding domain. We confirmed this through biophysical assays and by determining cryo-EM structures of spike protein ectodomains bound to 2 representative potent neutralizing antibody fragments. | ||
| + | |||
| + | Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies.,Zhu X, Mannar D, Srivastava SS, Berezuk AM, Demers JP, Saville JW, Leopold K, Li W, Dimitrov DS, Tuttle KS, Zhou S, Chittori S, Subramaniam S PLoS Biol. 2021 Apr 29;19(4):e3001237. doi: 10.1371/journal.pbio.3001237., eCollection 2021 Apr. PMID:33914735<ref>PMID:33914735</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7mjl" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Antibody 3D structures|Antibody 3D structures]] | ||
| + | *[[Spike protein 3D structures|Spike protein 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Severe acute respiratory syndrome coronavirus 2]] |
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Berezuk AM]] | ||
| + | [[Category: Chittori S]] | ||
| + | [[Category: Demers JP]] | ||
| + | [[Category: Dimitrov DS]] | ||
| + | [[Category: Leopold K]] | ||
| + | [[Category: Li W]] | ||
| + | [[Category: Mannar D]] | ||
| + | [[Category: Saville JW]] | ||
| + | [[Category: Srivastava SS]] | ||
| + | [[Category: Subramaniam S]] | ||
| + | [[Category: Tuttle KS]] | ||
| + | [[Category: Zhou S]] | ||
| + | [[Category: Zhu X]] | ||
Current revision
Cryo-EM structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain bound to Fab ab1 (focused refinement of RBD and Fab ab1)
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