7skw
From Proteopedia
(Difference between revisions)
| Line 4: | Line 4: | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7skw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SKW FirstGlance]. <br> | <table><tr><td colspan='2'>[[7skw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SKW FirstGlance]. <br> | ||
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 0.87Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7skw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7skw OCA], [https://pdbe.org/7skw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7skw RCSB], [https://www.ebi.ac.uk/pdbsum/7skw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7skw ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7skw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7skw OCA], [https://pdbe.org/7skw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7skw RCSB], [https://www.ebi.ac.uk/pdbsum/7skw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7skw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | |
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
Structures of two globular proteins were determined ab initio using microcrystal electron diffraction (MicroED) data that were collected on a direct electron detector in counting mode. Microcrystals were identified using a scanning electron microscope (SEM) and thinned with a focused ion beam (FIB) to produce crystalline lamellae of ideal thickness. Continuous-rotation data were collected using an ultra-low exposure rate to enable electron counting in diffraction. For the first sample, triclinic lysozyme extending to a resolution of 0.87 A, an ideal helical fragment of only three alanine residues provided initial phases. These phases were improved using density modification, allowing the entire atomic structure to be built automatically. A similar approach was successful on a second macromolecular sample, proteinase K, which is much larger and diffracted to a resolution of 1.5 A. These results demonstrate that macromolecules can be determined to sub-angstrom resolution by MicroED and that ab initio phasing can be successfully applied to counting data. | Structures of two globular proteins were determined ab initio using microcrystal electron diffraction (MicroED) data that were collected on a direct electron detector in counting mode. Microcrystals were identified using a scanning electron microscope (SEM) and thinned with a focused ion beam (FIB) to produce crystalline lamellae of ideal thickness. Continuous-rotation data were collected using an ultra-low exposure rate to enable electron counting in diffraction. For the first sample, triclinic lysozyme extending to a resolution of 0.87 A, an ideal helical fragment of only three alanine residues provided initial phases. These phases were improved using density modification, allowing the entire atomic structure to be built automatically. A similar approach was successful on a second macromolecular sample, proteinase K, which is much larger and diffracted to a resolution of 1.5 A. These results demonstrate that macromolecules can be determined to sub-angstrom resolution by MicroED and that ab initio phasing can be successfully applied to counting data. | ||
| - | Ab initio phasing macromolecular structures using electron-counted MicroED data.,Martynowycz MW, Clabbers MTB, Hattne J, Gonen T Nat Methods. 2022 Jun;19(6):724-729. doi: 10.1038/s41592-022-01485-4. Epub 2022, May 30. PMID:35637302<ref>PMID:35637302</ref> | + | Ab initio phasing macromolecular structures using electron-counted MicroED data.,Martynowycz MW, Clabbers MTB, Hattne J, Gonen T Nat Methods. 2022 Jun;19(6):724-729. doi: 10.1038/s41592-022-01485-4. Epub 2022 , May 30. PMID:35637302<ref>PMID:35637302</ref> |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 7skw" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 7skw" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Lysozyme 3D structures|Lysozyme 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
| Line 25: | Line 28: | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | + | [[Category: Clabbers MTB]] | |
| - | [[Category: Clabbers | + | [[Category: Gonen T]] |
| - | [[Category: Gonen | + | [[Category: Hattne J]] |
| - | [[Category: Hattne | + | [[Category: Martynowycz MW]] |
| - | [[Category: Martynowycz | + | |
| - | + | ||
Current revision
Ab initio structure of triclinic lysozyme from electron-counted MicroED data
| |||||||||||
