7u6r
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7u6r]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bat_coronavirus Bat coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U6R FirstGlance]. <br> | <table><tr><td colspan='2'>[[7u6r]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bat_coronavirus Bat coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U6R FirstGlance]. <br> | ||
- | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.5Å</td></tr> |
+ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u6r OCA], [https://pdbe.org/7u6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u6r RCSB], [https://www.ebi.ac.uk/pdbsum/7u6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u6r ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u6r OCA], [https://pdbe.org/7u6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u6r RCSB], [https://www.ebi.ac.uk/pdbsum/7u6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u6r ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/A0A1W6ASU7_9NIDO A0A1W6ASU7_9NIDO] | [https://www.uniprot.org/uniprot/A0A1W6ASU7_9NIDO A0A1W6ASU7_9NIDO] | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Middle East respiratory syndrome coronavirus (MERS-CoV) and several bat coronaviruses use dipeptidyl peptidase-4 (DPP4) as an entry receptor(1-4). However, the receptor for NeoCoV-the closest known MERS-CoV relative found in bats-remains unclear(5). Here, using a pseudotype virus entry assay, we found that NeoCoV and its close relative, PDF-2180, can efficiently bind to and use specific bat angiotensin-converting enzyme 2 (ACE2) orthologues and, less favourably, human ACE2 as entry receptors through their receptor-binding domains (RBDs) on the spike (S) proteins. Cryo-electron microscopy analysis revealed an RBD-ACE2 binding interface involving protein-glycan interactions, distinct from those of other known ACE2-using coronaviruses. We identified residues 337-342 of human ACE2 as a molecular determinant restricting NeoCoV entry, whereas a NeoCoV S pseudotyped virus containing a T510F RBD mutation efficiently entered cells expressing human ACE2. Although polyclonal SARS-CoV-2 antibodies or MERS-CoV RBD-specific nanobodies did not cross-neutralize NeoCoV or PDF-2180, an ACE2-specific antibody and two broadly neutralizing betacoronavirus antibodies efficiently inhibited these two pseudotyped viruses. We describe MERS-CoV-related viruses that use ACE2 as an entry receptor, underscoring a promiscuity of receptor use and a potential zoonotic threat. | ||
+ | |||
+ | Close relatives of MERS-CoV in bats use ACE2 as their functional receptors.,Xiong Q, Cao L, Ma C, Tortorici MA, Liu C, Si J, Liu P, Gu M, Walls AC, Wang C, Shi L, Tong F, Huang M, Li J, Zhao C, Shen C, Chen Y, Zhao H, Lan K, Corti D, Veesler D, Wang X, Yan H Nature. 2022 Dec;612(7941):748-757. doi: 10.1038/s41586-022-05513-3. Epub 2022 , Dec 7. PMID:36477529<ref>PMID:36477529</ref> | ||
+ | |||
+ | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
+ | </div> | ||
+ | <div class="pdbe-citations 7u6r" style="background-color:#fffaf0;"></div> | ||
+ | == References == | ||
+ | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> |
Current revision
Cryo-EM structure of PDF-2180 Spike glycoprotein
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