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Publication Abstract from PubMed

The cell cycle checkpoint kinase Mec1(ATR) and its integral partner Ddc2(ATRIP) are vital for the DNA damage and replication stress response. Mec1-Ddc2 "senses" single-stranded DNA (ssDNA) by being recruited to the ssDNA binding Replication Protein A (RPA) via Ddc2. In this study, we show that a DNA damage-induced phosphorylation circuit modulates checkpoint recruitment and function. We demonstrate that Ddc2-RPA interactions modulate the association between RPA and ssDNA and that Rfa1-phosphorylation aids in the further recruitment of Mec1-Ddc2. We also uncover an underappreciated role for Ddc2 phosphorylation that enhances its recruitment to RPA-ssDNA that is important for the DNA damage checkpoint in yeast. The crystal structure of a phosphorylated Ddc2 peptide in complex with its RPA interaction domain provides molecular details of how checkpoint recruitment is enhanced, which involves Zn(2+). Using electron microscopy and structural modeling approaches, we propose that Mec1-Ddc2 complexes can form higher order assemblies with RPA when Ddc2 is phosphorylated. Together, our results provide insight into Mec1 recruitment and suggest that formation of supramolecular complexes of RPA and Mec1-Ddc2, modulated by phosphorylation, would allow for rapid clustering of damage foci to promote checkpoint signaling.

A DNA damage-induced phosphorylation circuit enhances Mec1(ATR) Ddc2(ATRIP) recruitment to Replication Protein A.,Yates LA, Tannous EA, Morgan RM, Burgers PM, Zhang X Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2300150120. doi: , 10.1073/pnas.2300150120. Epub 2023 Mar 30. PMID:36996117^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.