1tg7
From Proteopedia
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[[Image:1tg7.gif|left|200px]] | [[Image:1tg7.gif|left|200px]] | ||
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'''Native structure of beta-galactosidase from Penicillium sp.''' | '''Native structure of beta-galactosidase from Penicillium sp.''' | ||
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[[Category: Polikarpov, I.]] | [[Category: Polikarpov, I.]] | ||
[[Category: Rojas, A L.]] | [[Category: Rojas, A L.]] | ||
| - | [[Category: | + | [[Category: Family gh35]] |
| - | [[Category: | + | [[Category: Glycoprotein]] |
| - | [[Category: | + | [[Category: Glycoside hydrolase]] |
| - | [[Category: | + | [[Category: Penicillium]] |
| - | [[Category: | + | [[Category: Tim barrel domain]] |
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Revision as of 06:55, 3 May 2008
Native structure of beta-galactosidase from Penicillium sp.
Overview
Beta-galactosidases catalyze the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. Here we report the crystallographic structures of Penicillium sp. beta-galactosidase and its complex with galactose solved by the SIRAS quick cryo-soaking technique at 1.90 A and 2.10 A resolution, respectively. The amino acid sequence of this 120 kDa protein was first assigned putatively on the basis of inspection of the experimental electron density maps and then determined by nucleotide sequence analysis. Primary structure alignments reveal that Penicillium sp. beta-galactosidase belongs to family 35 of glycosyl hydrolases (GHF-35). This model is the first 3D structure for a member of GHF-35. Five distinct domains which comprise the structure are assembled in a way previously unobserved for beta-galactosidases. Superposition of this complex with other beta-galactosidase complexes from several hydrolase families allowed the identification of residue Glu200 as the proton donor and residue Glu299 as the nucleophile involved in catalysis. Penicillium sp. beta-galactosidase is a glycoprotein containing seven N-linked oligosaccharide chains and is the only structure of a glycosylated beta-galactosidase described to date.
About this Structure
1TG7 is a Single protein structure of sequence from Penicillium sp.. Full crystallographic information is available from OCA.
Reference
Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose., Rojas AL, Nagem RA, Neustroev KN, Arand M, Adamska M, Eneyskaya EV, Kulminskaya AA, Garratt RC, Golubev AM, Polikarpov I, J Mol Biol. 2004 Nov 5;343(5):1281-92. PMID:15491613 Page seeded by OCA on Sat May 3 09:55:01 2008
Categories: Beta-galactosidase | Penicillium sp. | Single protein | Adamska, M. | Arand, M. | Eneyskaya, E V. | Garratt, R C. | Golubev, A M. | Kulminskaya, A A. | Nagem, R A.P. | Neustroev, K N. | Polikarpov, I. | Rojas, A L. | Family gh35 | Glycoprotein | Glycoside hydrolase | Penicillium | Tim barrel domain
