|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Human Regenerating Gene Type IV (REG IV) PROTEIN, P91S mutant== | | ==Human Regenerating Gene Type IV (REG IV) PROTEIN, P91S mutant== |
| - | <StructureSection load='2kv3' size='340' side='right'caption='[[2kv3]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2kv3' size='340' side='right'caption='[[2kv3]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2kv3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KV3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KV3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2kv3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KV3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KV3 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">REG4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kv3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kv3 OCA], [https://pdbe.org/2kv3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kv3 RCSB], [https://www.ebi.ac.uk/pdbsum/2kv3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kv3 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kv3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kv3 OCA], [https://pdbe.org/2kv3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kv3 RCSB], [https://www.ebi.ac.uk/pdbsum/2kv3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kv3 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/REG4_HUMAN REG4_HUMAN]] Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.<ref>PMID:12819006</ref> <ref>PMID:20692269</ref>
| + | [https://www.uniprot.org/uniprot/REG4_HUMAN REG4_HUMAN] Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.<ref>PMID:12819006</ref> <ref>PMID:20692269</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 14: |
Line 14: |
| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kv/2kv3_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kv/2kv3_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
| Line 32: |
Line 32: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chen, C]] | + | [[Category: Chen C]] |
| - | [[Category: Ho, M]] | + | [[Category: Ho M]] |
| - | [[Category: Lou, Y]] | + | [[Category: Lou Y]] |
| - | [[Category: C-type lectin]]
| + | |
| - | [[Category: Disulfide bond]]
| + | |
| - | [[Category: Gisp]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Lectin]]
| + | |
| - | [[Category: Reg 4]]
| + | |
| - | [[Category: Reg iv]]
| + | |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
Function
REG4_HUMAN Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Human RegIV protein, which contains a sequence motif homologous to calcium-dependent (C-type) lectin-like domain, is highly expressed in mucosa cells of the gastrointestinal tract during pathogen infection and carcinogenesis and may be applied in both diagnosis and treatment of gastric and colon cancers. Here, we provide evidence that, unlike other C-type lectins, human RegIV binds to polysaccharides, mannan, and heparin in the absence of calcium. To elucidate the structural basis for carbohydrate recognition by NMR, we generated the mutant with Pro91 replaced by Ser (hRegIV-P91S) and showed that the structural property and carbohydrate binding ability of hRegIV-P91S are almost identical with those of wild-type protein. The solution structure of hRegIV-P91S was determined, showing that it adopts a typical fold of C-type lectin. Based on the chemical shift perturbations of amide resonances, two calcium-independent mannan-binding sites were proposed. One site is similar to the calcium-independent sugar-binding site on human RegIII and Langerin. Interestingly, the other site is adjacent to the conserved calcium-dependent site at position Ca-2 of typical C-type lectins. Moreover, model-free analysis of (15)N relaxation parameters and simplified Carr-Purcell-Meiboom-Gill relaxation dispersion experiments showed that a slow microsecond-to-millisecond time-scale backbone motion is involved in mannan binding by this site, suggesting a potential role for specific carbohydrate recognition. Our findings shed light on the sugar-binding mode of Reg family proteins, and we postulate that Reg family proteins evolved to bind sugar without calcium to keep the carbohydrate recognition activity under low-pH environments in the gastrointestinal tract.
Human RegIV protein adopts a typical C-type lectin fold but binds mannan with two calcium-independent sites.,Ho MR, Lou YC, Wei SY, Luo SC, Lin WC, Lyu PC, Chen C J Mol Biol. 2010 Oct 1;402(4):682-95. Epub 2010 Aug 6. PMID:20692269[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kamarainen M, Heiskala K, Knuutila S, Heiskala M, Winqvist O, Andersson LC. RELP, a novel human REG-like protein with up-regulated expression in inflammatory and metaplastic gastrointestinal mucosa. Am J Pathol. 2003 Jul;163(1):11-20. PMID:12819006 doi:http://dx.doi.org/10.1016/S0002-9440(10)63625-5
- ↑ Ho MR, Lou YC, Wei SY, Luo SC, Lin WC, Lyu PC, Chen C. Human RegIV protein adopts a typical C-type lectin fold but binds mannan with two calcium-independent sites. J Mol Biol. 2010 Oct 1;402(4):682-95. Epub 2010 Aug 6. PMID:20692269 doi:http://dx.doi.org/10.1016/j.jmb.2010.07.061
- ↑ Ho MR, Lou YC, Wei SY, Luo SC, Lin WC, Lyu PC, Chen C. Human RegIV protein adopts a typical C-type lectin fold but binds mannan with two calcium-independent sites. J Mol Biol. 2010 Oct 1;402(4):682-95. Epub 2010 Aug 6. PMID:20692269 doi:http://dx.doi.org/10.1016/j.jmb.2010.07.061
|
