2w7y

From Proteopedia

(Difference between revisions)

Current revision

Structure of a Streptococcus pneumoniae solute-binding protein in complex with the blood group A-trisaccharide.

Structural highlights

2w7y is a 2 chain structure with sequence from Streptococcus pneumoniae SP3-BS71. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.35Å
Ligands:	, , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Streptococcus pneumoniae is a common bacterial pathogen that is well known for its ability to cause acute respiratory disease (pneumonia), ear infections, and other serious illnesses. This Gram-positive bacterium relies on its carbohydrate-metabolizing capabilities for full virulence in its host; however, the range of glycan targets that it can attack is presently not fully appreciated. S. pneumoniae is known to have a fucose utilization operon that in the TIGR4 strain plays a role in its virulence. Here we identify a second type of fucose utilization operon that is present in a subset of S. pneumoniae strains, including the serotype 3 strain SP3-BS71. This operon contains a transporter with a solute-binding protein, FcsSBP (fucose solute-binding protein), that interacts tightly (Ka approximately 1 x 10(6) M(-1)) and specifically with soluble A- and B-antigen trisaccharides but displays no selectivity between these two sugars. The structure of the FcsSBP in complex with the A-trisaccharide antigen, determined to 2.35 A, reveals its mode of binding to the reducing end of this sugar, thus highlighting this protein's requirement for soluble blood group antigen ligands. Overall, this report exposes a heretofore unknown capability of certain S. pneumoniae strains to transport and potentially metabolize the histo-blood group antigen carbohydrates of its host.

Blood group antigen recognition by a solute-binding protein from a serotype 3 strain of Streptococcus pneumoniae.,Higgins MA, Abbott DW, Boulanger MJ, Boraston AB J Mol Biol. 2009 May 1;388(2):299-309. Epub 2009 Mar 12. PMID:19285508^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Higgins MA, Abbott DW, Boulanger MJ, Boraston AB. Blood group antigen recognition by a solute-binding protein from a serotype 3 strain of Streptococcus pneumoniae. J Mol Biol. 2009 May 1;388(2):299-309. Epub 2009 Mar 12. PMID:19285508 doi:10.1016/j.jmb.2009.03.012

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2w7y"

@@ Line 3: / Line 3: @@
 <StructureSection load='2w7y' size='340' side='right'caption='[[2w7y]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2w7y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_sp3-bs71 Streptococcus pneumoniae sp3-bs71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W7Y FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2w7y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_SP3-BS71 Streptococcus pneumoniae SP3-BS71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W7Y FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w7y OCA], [https://pdbe.org/2w7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w7y RCSB], [https://www.ebi.ac.uk/pdbsum/2w7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w7y ProSAT]</span></td></tr>
 </table>
@@ Line 13: / Line 13: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w7/2w7y_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 35: / Line 35: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Streptococcus pneumoniae sp3-bs71]]
+[[Category: Streptococcus pneumoniae SP3-BS71]]
-[[Category: Abbott, D W]]
+[[Category: Abbott DW]]
-[[Category: Boraston, A B]]
+[[Category: Boraston AB]]
-[[Category: Boulanger, M J]]
+[[Category: Boulanger MJ]]
-[[Category: Higgins, M A]]
+[[Category: Higgins MA]]
-[[Category: Blood group antigen]]
-[[Category: Carbohydrate transport]]
-[[Category: Solute-binding protein]]
-[[Category: Streptococcus pneumoniae]]
-[[Category: Sugar binding protein]]
-[[Category: Sugar-binding protein]]

2w7y

From Proteopedia

Current revision

Structure of a Streptococcus pneumoniae solute-binding protein in complex with the blood group A-trisaccharide.

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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