1tj7

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[[Image:1tj7.gif|left|200px]]
[[Image:1tj7.gif|left|200px]]
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{{Structure
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|PDB= 1tj7 |SIZE=350|CAPTION= <scene name='initialview01'>1tj7</scene>, resolution 2.44&Aring;
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The line below this paragraph, containing "STRUCTURE_1tj7", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1tj7| PDB=1tj7 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tj7 OCA], [http://www.ebi.ac.uk/pdbsum/1tj7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tj7 RCSB]</span>
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}}
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'''Structure determination and refinement at 2.44 A resolution of Argininosuccinate lyase from E. coli'''
'''Structure determination and refinement at 2.44 A resolution of Argininosuccinate lyase from E. coli'''
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[[Category: Koski, M K.]]
[[Category: Koski, M K.]]
[[Category: Wierenga, R K.]]
[[Category: Wierenga, R K.]]
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[[Category: argininosuccinate lyase]]
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[[Category: Argininosuccinate lyase]]
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[[Category: aspartase]]
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[[Category: Aspartase]]
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[[Category: crystallin]]
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[[Category: Crystallin]]
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[[Category: e. coli]]
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[[Category: E. coli]]
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[[Category: fumarase]]
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[[Category: Fumarase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:00:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:56:46 2008''
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Revision as of 07:00, 3 May 2008

Image:1tj7.gif

Template:STRUCTURE 1tj7

Structure determination and refinement at 2.44 A resolution of Argininosuccinate lyase from E. coli


Overview

Escherichia coli argininosuccinate lyase has been crystallized from a highly concentrated sample of purified recombinant alpha-methylacyl-CoA racemase, in which it occurred as a minor impurity. The structure has been solved using molecular replacement at 2.44 A resolution. The enzyme is tetrameric, but in this crystal form there is a dimer in the asymmetric unit. The tetramer has four active sites; each active site is constructed from loops of three different subunits. One of these catalytic loops, near residues Ser277 and Ser278, was disordered in previous structures of active lyases, but is very well ordered in this structure in one of the subunits owing to the presence of two phosphate ions in the active-site cavity. The positions of these phosphate ions indicate a plausible mode of binding of the succinate moiety of the substrate in the competent catalytic complex.

About this Structure

1TJ7 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure determination and refinement at 2.44 A resolution of argininosuccinate lyase from Escherichia coli., Bhaumik P, Koski MK, Bergmann U, Wierenga RK, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):1964-70. Epub 2004, Oct 20. PMID:15502303 Page seeded by OCA on Sat May 3 10:00:41 2008

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