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| | <StructureSection load='3zh6' size='340' side='right'caption='[[3zh6]], [[Resolution|resolution]] 2.29Å' scene=''> | | <StructureSection load='3zh6' size='340' side='right'caption='[[3zh6]], [[Resolution|resolution]] 2.29Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3zh6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZH6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3zh6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZH6 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.292Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3zh5|3zh5]], [[3zh7|3zh7]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zh6 OCA], [https://pdbe.org/3zh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zh6 RCSB], [https://www.ebi.ac.uk/pdbsum/3zh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zh6 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zh6 OCA], [https://pdbe.org/3zh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zh6 RCSB], [https://www.ebi.ac.uk/pdbsum/3zh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zh6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacterium influenzae lehmann and neumann 1896]] | + | [[Category: Haemophilus influenzae]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Al-Jubair, T]] | + | [[Category: Al-Jubair T]] |
| - | [[Category: Riesbeck, K]] | + | [[Category: Riesbeck K]] |
| - | [[Category: Singh, B]] | + | [[Category: Singh B]] |
| - | [[Category: Thunnissen, M M.G M]] | + | [[Category: Thunnissen MMGM]] |
| - | [[Category: Cell adhesion]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Haemophilus influenzae protein E (PE) is a multifunctional adhesin involved in direct interactions with lung epithelial cells and host proteins, including plasminogen and the extracellular matrix proteins vitronectin and laminin. We recently crystallized PE and successfully collected X-ray diffraction data at 1.8 A. Here, we solved the structure of a recombinant version of PE and analyzed different functional regions. It is a dimer in solution and in the asymmetric unit of the crystals. The dimer has a structure that resembles a flattened beta-barrel. It is, however, not a true beta-barrel, as there are differences in both the hydrogen-bonding pattern and the shape. Each monomer consisted of a 6-stranded antiparallel beta-sheet with a rigid alpha-helix at the C terminus tethered to the concave side of the sheet by a disulfide bridge. The laminin/plasminogen binding region (residues 41 to 68) is exposed, while the vitronectin binding region (residues 84 to 108) is partially accessible in the dimer. The dimerized PE explains the simultaneous interaction with laminin and vitronectin. In addition, we found this unique adhesin to be present in many bacterial genera of the family Pasteurellaceae and also orthologues in other, unrelated species (Enterobacter cloacae and Listeria monocytogenes). Peptides corresponding to the surface-exposed regions PE 24 to 37, PE 74 to 89, and PE 134 to 156 were immunogenic in the mouse. Importantly, these peptide-based antibodies also recognized PE at the bacterial surface. Taken together, our detailed structure of PE explains how this important virulence factor of H. influenzae simultaneously interacts with host vitronectin, laminin, or plasminogen, promoting bacterial pathogenesis.
The unique structure of Haemophilus influenzae protein E reveals multiple binding sites for host factors.,Singh B, Al-Jubair T, Morgelin M, Thunnissen MM, Riesbeck K Infect Immun. 2013 Mar;81(3):801-14. doi: 10.1128/IAI.01111-12. Epub 2012 Dec 28. PMID:23275089[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Singh B, Al-Jubair T, Morgelin M, Thunnissen MM, Riesbeck K. The unique structure of Haemophilus influenzae protein E reveals multiple binding sites for host factors. Infect Immun. 2013 Mar;81(3):801-14. doi: 10.1128/IAI.01111-12. Epub 2012 Dec 28. PMID:23275089 doi:10.1128/IAI.01111-12
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