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| | <StructureSection load='5w0t' size='340' side='right'caption='[[5w0t]], [[Resolution|resolution]] 2.63Å' scene=''> | | <StructureSection load='5w0t' size='340' side='right'caption='[[5w0t]], [[Resolution|resolution]] 2.63Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5w0t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W0T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5w0t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W0T FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.63Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MSP1, YTA4, YGR028W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w0t OCA], [https://pdbe.org/5w0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w0t RCSB], [https://www.ebi.ac.uk/pdbsum/5w0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w0t ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w0t OCA], [http://pdbe.org/5w0t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w0t RCSB], [http://www.ebi.ac.uk/pdbsum/5w0t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w0t ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MSP1_YEAST MSP1_YEAST]] Involved in intramitochondrial sorting of proteins. | + | [https://www.uniprot.org/uniprot/MSP1_YEAST MSP1_YEAST] Involved in intramitochondrial sorting of proteins. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Keenan, R J]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Mateja, A M]] | + | [[Category: Keenan RJ]] |
| - | [[Category: Wohlever, M L]] | + | [[Category: Mateja AM]] |
| - | [[Category: Aaa atpase]] | + | [[Category: Wohlever ML]] |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
MSP1_YEAST Involved in intramitochondrial sorting of proteins.
Publication Abstract from PubMed
Mislocalized tail-anchored (TA) proteins of the outer mitochondrial membrane are cleared by a newly identified quality control pathway involving the conserved eukaryotic protein Msp1 (ATAD1 in humans). Msp1 is a transmembrane AAA-ATPase, but its role in TA protein clearance is not known. Here, using purified components reconstituted into proteoliposomes, we show that Msp1 is both necessary and sufficient to drive the ATP-dependent extraction of TA proteins from the membrane. A crystal structure of the Msp1 cytosolic region modeled into a ring hexamer suggests that active Msp1 contains a conserved membrane-facing surface adjacent to a central pore. Structure-guided mutagenesis of the pore residues shows that they are critical for TA protein extraction in vitro and for functional complementation of an msp1 deletion in yeast. Together, these data provide a molecular framework for Msp1-dependent extraction of mislocalized TA proteins from the outer mitochondrial membrane.
Msp1 Is a Membrane Protein Dislocase for Tail-Anchored Proteins.,Wohlever ML, Mateja A, McGilvray PT, Day KJ, Keenan RJ Mol Cell. 2017 Jul 20;67(2):194-202.e6. doi: 10.1016/j.molcel.2017.06.019. Epub, 2017 Jul 14. PMID:28712723[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wohlever ML, Mateja A, McGilvray PT, Day KJ, Keenan RJ. Msp1 Is a Membrane Protein Dislocase for Tail-Anchored Proteins. Mol Cell. 2017 Jul 20;67(2):194-202.e6. doi: 10.1016/j.molcel.2017.06.019. Epub, 2017 Jul 14. PMID:28712723 doi:http://dx.doi.org/10.1016/j.molcel.2017.06.019
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