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| | <StructureSection load='6n1a' size='340' side='right'caption='[[6n1a]], [[Resolution|resolution]] 1.60Å' scene=''> | | <StructureSection load='6n1a' size='340' side='right'caption='[[6n1a]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6n1a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_plauti"_(sic)_seguin_1928 "bacillus plauti" (sic) seguin 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N1A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6n1a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Flavonifractor_plautii Flavonifractor plautii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N1A FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">A4U99_12080 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=292800 "Bacillus plauti" (sic) Seguin 1928])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n1a OCA], [https://pdbe.org/6n1a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n1a RCSB], [https://www.ebi.ac.uk/pdbsum/6n1a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n1a ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n1a OCA], [http://pdbe.org/6n1a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n1a RCSB], [http://www.ebi.ac.uk/pdbsum/6n1a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n1a ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Flavonifractor plautii]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Rahfeld, P]] | + | [[Category: Rahfeld P]] |
| - | [[Category: Sim, L]] | + | [[Category: Sim L]] |
| - | [[Category: Withers, S G]] | + | [[Category: Withers SG]] |
| - | [[Category: A blood antigen]]
| + | |
| - | [[Category: Cazy]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: N-acetylgalactosamine deacetylase]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Access to efficient enzymes that can convert A and B type red blood cells to 'universal' donor O would greatly increase the supply of blood for transfusions. Here we report the functional metagenomic screening of the human gut microbiome for enzymes that can remove the cognate A and B type sugar antigens. Among the genes encoded in our library of 19,500 expressed fosmids bearing gut bacterial DNA, we identify an enzyme pair from the obligate anaerobe Flavonifractor plautii that work in concert to efficiently convert the A antigen to the H antigen of O type blood, via a galactosamine intermediate. The X-ray structure of the N-acetylgalactosamine deacetylase reveals the active site and mechanism of the founding member of an esterase family. The galactosaminidase expands activities within the CAZy family GH36. Their ability to completely convert A to O of the same rhesus type at very low enzyme concentrations in whole blood will simplify their incorporation into blood transfusion practice, broadening blood supply.
An enzymatic pathway in the human gut microbiome that converts A to universal O type blood.,Rahfeld P, Sim L, Moon H, Constantinescu I, Morgan-Lang C, Hallam SJ, Kizhakkedathu JN, Withers SG Nat Microbiol. 2019 Jun 10. pii: 10.1038/s41564-019-0469-7. doi:, 10.1038/s41564-019-0469-7. PMID:31182795[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Rahfeld P, Sim L, Moon H, Constantinescu I, Morgan-Lang C, Hallam SJ, Kizhakkedathu JN, Withers SG. An enzymatic pathway in the human gut microbiome that converts A to universal O type blood. Nat Microbiol. 2019 Jun 10. pii: 10.1038/s41564-019-0469-7. doi:, 10.1038/s41564-019-0469-7. PMID:31182795 doi:http://dx.doi.org/10.1038/s41564-019-0469-7
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