7t32
From Proteopedia
(Difference between revisions)
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==CryoEM structure of the adenosine 2A receptor-BRIL/Anti BRIL Fab complex with ZM241385== | ==CryoEM structure of the adenosine 2A receptor-BRIL/Anti BRIL Fab complex with ZM241385== | ||
- | <StructureSection load='7t32' size='340' side='right'caption='[[7t32]]' scene=''> | + | <StructureSection load='7t32' size='340' side='right'caption='[[7t32]], [[Resolution|resolution]] 3.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
- | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T32 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7t32]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T32 FirstGlance]. <br> |
- | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t32 OCA], [https://pdbe.org/7t32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t32 RCSB], [https://www.ebi.ac.uk/pdbsum/7t32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t32 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> |
+ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZMA:4-{2-[(7-AMINO-2-FURAN-2-YL[1,2,4]TRIAZOLO[1,5-A][1,3,5]TRIAZIN-5-YL)AMINO]ETHYL}PHENOL'>ZMA</scene></td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t32 OCA], [https://pdbe.org/7t32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t32 RCSB], [https://www.ebi.ac.uk/pdbsum/7t32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t32 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Single particle cryogenic-electron microscopy (cryo-EM) is used extensively to determine structures of activated G protein-coupled receptors (GPCRs) in complex with G proteins or arrestins. However, applying it to GPCRs without signaling proteins remains challenging because most receptors lack structural features in their soluble domains to facilitate image alignment. In GPCR crystallography, inserting a fusion protein between transmembrane helices 5 and 6 is a highly successful strategy for crystallization. Although a similar strategy has the potential to broadly facilitate cryo-EM structure determination of GPCRs alone without signaling protein, the critical determinants that make this approach successful are not yet clear. Here, we address this shortcoming by exploring different fusion protein designs, which lead to structures of antagonist bound A(2A) adenosine receptor at 3.4 A resolution and unliganded Smoothened at 3.7 A resolution. The fusion strategies explored here are likely applicable to cryo-EM interrogation of other GPCRs and small integral membrane proteins. | ||
+ | |||
+ | Fusion protein strategies for cryo-EM study of G protein-coupled receptors.,Zhang K, Wu H, Hoppe N, Manglik A, Cheng Y Nat Commun. 2022 Jul 28;13(1):4366. doi: 10.1038/s41467-022-32125-2. PMID:35902590<ref>PMID:35902590</ref> | ||
+ | |||
+ | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
+ | </div> | ||
+ | <div class="pdbe-citations 7t32" style="background-color:#fffaf0;"></div> | ||
+ | |||
+ | ==See Also== | ||
+ | *[[Adenosine A2A receptor 3D structures|Adenosine A2A receptor 3D structures]] | ||
+ | == References == | ||
+ | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
+ | [[Category: Escherichia coli]] | ||
+ | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cheng YF]] | [[Category: Cheng YF]] |
Current revision
CryoEM structure of the adenosine 2A receptor-BRIL/Anti BRIL Fab complex with ZM241385
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Categories: Escherichia coli | Homo sapiens | Large Structures | Cheng YF | Hoppe N | Manglik A | Wu H | Zhang KH